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Novel fluorescence immunoassay for the detection of zearalenone using HRP-mediated fluorescence quenching of gold-silver bimetallic nanoclusters

文献类型： 外文期刊

作者： Liu, Zhenjiang 1 ; Wang, Xiaoyan 1 ; Ren, Xuexiang 3 ; Li, Weibin 1 ; Sun, Jianfan 1 ; Wang, Xinwei 1 ; Huang, Yuqian; 1 ;

作者机构： 1.Jiangsu Univ, Sch Environm & Safety Engn, Inst Environm & Ecol, Zhenjiang 212013, Jiangsu, Peoples R China

2.Hainan Normal Univ, Minist Educ, Key Lab Trop Med Resource Chem, Haikou, Hainan, Peoples R China

3.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei 230001, Peoples R China

4.Huaibei Normal Univ, Coll Life Sci, Huaibei 235099, Peoples R China

关键词： Ratiometric fluorescence ELISA; Gold-silver bimetallic nanoclusters; Horseradish peroxidase; Zearalenone

期刊名称：FOOD CHEMISTRY （ 影响因子：6.306； 五年影响因子：6.219 ）

ISSN： 0308-8146

年卷期： 2021 年 355 卷

页码：

收录情况： SCI

摘要： In the presented study, a horseradish peroxidase (HRP)-mediated ratiometric fluorescence enzyme-linked immunosorbent assay (ELISA) for zearalenone (ZEN) was reported based on fluorescence quenching of goldsilver bimetallic nanoclusters (Au-Ag NCs). HRP-antibody was used as a bridge in this immunoassay, linking the ratiometric fluorescence signal to the ZEN concentration. HRP catalyzed the oxidization of o-phenylenediamine in the presence of H2O2, leading to the formation of 2,3-diaminophenazine, which not only delivered a new peak at 580 nm but also quenched Au-Ag NCs fluorescence at 690 nm. Under optimal conditions, the detection limit for the proposed ELISA was 0.017 ng/mL, which was approximately 6.6-fold lower than conventional ELISA. Moreover, analytical performances were evaluated fully including specificity, accuracy, precision, and practicability, and showed that this method provides a potential platform for sensitive and reliable detection of ZEN.

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