您好，欢迎访问安徽省农业科学院 机构知识库！

Discovery of a key gene associated with fruit maturity date and analysis of its regulatory pathway in peach

文献类型： 外文期刊

作者： Cao, Ke 1 ; Pan, Haifa 3 ; Zhao, Yalin 1 ; Bie, Hangling 1 ; Wang, Jiao 1 ; Zhu, Gengrui 1 ; Fang, Weichao 1 ; Chen, Changwen 1 ; Wang, Xinwei 1 ; Li, Yong 1 ; Wu, Jinlong 1 ; Khan, Irshad Ahmad 1 ; Zhang, Jinyun 3 ; Wang, Lirong 1 ;

作者机构： 1.Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Key Lab Biol & Genet Improvement Hort Crops Fruit, Minist Agr, Zhengzhou 450009, Peoples R China

2.Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Zhengzhou 450009, Peoples R China

3.Anhui Acad Agr Sci, Hort Res Inst, Hefei 230031, Peoples R China

关键词： Peach; Maturity date; Genome wide association analysis; NAC gene

期刊名称：PLANT SCIENCE （ 影响因子：5.2； 五年影响因子：5.7 ）

ISSN： 0168-9452

年卷期： 2023 年 333 卷

页码：

收录情况： SCI

摘要： Fruit maturity is an important agronomic trait of fruit crops. Although in previous studies, several molecular markers are developed for the trait, the knowledge about its candidate genes is particularly limited. In this study, a total of 357 peach accessions were re-sequenced to obtain 949,638 SNPs. Combing with 3-year fruit maturity dates, a genome-wide association analysis was performed, and 5, 8, and 9 association loci were identified. To screen the candidate genes for those year-stable loci on chromosomes 4 and 5, two maturity date mutants were used for transcriptome sequencing. Gene expression analysis indicated that Prupe.4G186800 and Pru-pe.4G187100 on chromosome 4 were essential to fruit ripening in peaches. However, the expression analysis of different tissues showed that the first gene has no tissue-specific character, but transgenic studies showed that the latter is more likely to be a key candidate gene than the first for the maturity date in peach. The yeast two-hybrid assay showed that the proteins encoded by the two genes interacted and then regulated fruit ripening. Moreover, the previously identified 9 bp insertion in Prupe.4G186800 may affect their interaction ability. This research is of great significance for understanding the molecular mechanism of peach fruit ripening and developing practical molecular markers in a breeding program.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台