miRNA novel_268 targeting NlABCG3 is involved in nitenpyram and clothianidin resistance in Nilaparvata lugens
文献类型： 外文期刊
作者： Li, Zhao 1 ; Mao, Kaikai 2 ; Jin, Ruoheng 2 ; Cai, Tingwei 2 ; Qin, Yao 2 ; Zhang, Yunhua 2 ; He, Shun 2 ; Ma, Kangsheng 2 ; Wan, Hu 2 ; Ren, Xuexiang 1 ; Li, Jianhong 2 ;
作者机构： 1.Anhui Acad Agr Sci, Inst Plant Protect & Agro Prod Safety, 40 Nongkenan Rd, Hefei 230031, Peoples R China
2.Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pest, Wuhan 430070, Peoples R China
关键词： NlABCG3; miRNA; Gene expression; Resistance; Nilaparvata lugens
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）
ISSN： 0141-8130
年卷期： 2022 年 217 卷
页码：
收录情况： SCI
摘要： The brown planthopper (BPH), Nilaparvata lugens (Stal), is one of the most destructive pests that seriously threatens the high-quality and safe production of rice. However, due to the unscientific use of chemical in-secticides, N. lugens has developed varying levels of resistance to insecticides, including nitenpyram and clo-thianidin. The ATP-binding cassette (ABC) transporter plays a nonnegligible role in phase III of the detoxification process, which may play an important role in insecticide resistance. In the present study, NlABCG3 was signif-icantly overexpressed in both the NR and CR populations compared with susceptible populations. Silencing NlABCG3 significantly increased the susceptibility of BPH to nitenpyram and clothianidin. In addition, RNAi-mediated knockdown of three key genes in the miRNA biogenesis pathway altered the level of NlABCG3. Sub-sequently, the luciferase reporter assays demonstrated that novel_268 binds to the NlABCG3 coding region and downregulates its expression. Furthermore, injection of miRNA inhibitors or mimics of novel_268 significantly altered the susceptibility of N. lugens to nitenpyram and clothianidin. These results suggest that miRNA novel_268 targeting NlABCG3 is involved in nitenpyram and clothianidin resistance in N. lugens. These findings may help to enhance our knowledge of the transcriptional regulation of the ABC transporter that mediate insecticide resistance in N. lugens.
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