文献类型： 外文期刊

作者： Hu, Wenping ¹ ; Dong, Xinlong ¹ ; Tian, Zhilong ¹ ; Zhang, Zhuangbiao ¹ ; Tang, Jishun ¹ ; Liang, Benmeng ¹ ; Liu, Qiu ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Key Lab Anim Genet & Breeding & Reprod, Minist Agr & Rural Affairs, Beijing 100193, Peoples R China

2.Anhui Acad Agr Sci, Inst Anim Husb & Vet Med, Hefei 230031, Peoples R China

3.Chinese Acad Sci, Innovat Acad Seed Design, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

关键词： Fertilization; Izumo1; Juno; Sheep; Single cell RNA-seq

期刊名称：JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY （ 影响因子：4.167； 五年影响因子：4.392 ）

ISSN： 1674-9782

年卷期： 2021 年 12 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundJUNO and IZUMO1 are the first receptor-ligand protein pairs discovered to be essential for sperm-oocyte fusion; their interaction is indispensable for fertilization.MethodsPCR was used to clone the full-length DNA sequence of the Juno gene in sheep. The single nucleotide polymorphism (SNP) loci of Juno were genotyped by Sequenom MassARRAY (R). PCR combined with rapid amplification of cDNA Ends were used to clone the full-length cDNA sequence of Juno and Izumo1. Reverse transcriptase-PCR (RT-PCR) and real time-quantitative-PCR (RT-qPCR) were used to analyze the genes' expression in tissues of sheep, and single cell RNA-seq was used to analyze the genes' expression in oocytes, granulosa cells and follicular theca of polytocous and monotocous Small Tail Han ewes. Bioinformatics was used to analyze advanced structure and phylogeny of JUNO and IZUMO1 proteins.ResultsThe full-length DNA sequence of the Juno gene in sheep was cloned and nine SNPs were screened. We found a significant association between the g.848253 C>A locus of Juno and litter size of Small Tail Han sheep (P<0.05). The full-length cDNA sequence of Juno and Izumo1 genes from Small Tail Han sheep were obtained. We found a new segment of the Izumo1 CDS consisting of 35bp, and we confirmed the Izumo1 gene has 9 exons, not 8. RT-qPCR showed that Juno and Izumo1 genes were highly expressed in ovarian and testicular tissues, respectively (P<0.01). Single cell RNA-seq showed Juno was specifically expressed in oocytes, but not in granulosa cells or follicular theca, while Izumo1 displayed little to no expression in all three cell types. There was no difference in expression of the Juno gene in oocyte and ovarian tissue in sheep with different litter sizes, indicating expression of Juno is not related to litter size traits. Bioinformatic analysis revealed the g.848253 C>A locus of Juno results in a nonconservative missense point mutation leading to a change from Phe to Leu at position 219 in the amino acid sequence.ConclusionsFor the first time, this study systematically analyzed the expression, structure and function of Juno and Izumo1 genes and their encoded proteins in Small Tail Han sheep, providing the basis for future studies of the regulatory mechanisms of Juno and Izumo1 genes.