Genomic Characterization of Phage ZP3 and Its Endolysin LysZP with Antimicrobial Potential against Xanthomonas oryzae pv. oryzae
文献类型： 外文期刊
作者： Zhang, Muchen 1 ; Xu, Xinyan 1 ; Lv, Luqiong 1 ; Luo, Jinyan 3 ; Ahmed, Temoor 1 ; Alsakkaf, Waleed A. A. 6 ; Ali, Hayssam M. 6 ; Bi, Ji'an 7 ; Yan, Chengqi 7 ; Gu, Chunyan 8 ; Shou, Linfei 9 ; Li, Bin 1 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, Minist Agr Key Lab Mol Biol Crop Pathogens & Insec, State Key Lab Rice Biol & Breeding,Zhejiang Key La, Hangzhou 310058, Peoples R China
2.Shanghai Ctr Agr Prod Qual Safety, Minist Agr & Rural Affairs Shanghai, Food Qual Supervis Inspect & Testing Ctr, Shanghai 201708, Peoples R China
3.Shanghai Extens & Serv Ctr Agr Technol, Dept Plant Quarantine, Shanghai 201103, Peoples R China
4.Western Caspian Univ, Dept Life Sci, AZ-1001 Baku, Azerbaijan
5.Middle East Univ, MEU Res Unit, Amman 11192, Jordan
6.King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia
7.Ningbo Acad Agr Sci, Crop Inst, Ningbo 315040, Peoples R China
8.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Anhui Prov Key Lab Pesticide Resistance Management, Hefei 230031, Peoples R China
9.Stn Plant Protect & Quarantine & Control Agrochem, Hangzhou 310004, Peoples R China
关键词： Xanthomonas oryzae pv. oryzae; phage isolation; endolysin; biocontrol
期刊名称：VIRUSES-BASEL （ 影响因子：3.5； 五年影响因子：3.7 ）
ISSN：
年卷期： 2024 年 16 卷 9 期
页码：
收录情况： SCI
摘要： Xanthomonas oryzae pv. oryzae (Xoo) is a significant bacterial pathogen responsible for outbreaks of bacterial leaf blight in rice, posing a major threat to rice cultivation worldwide. Effective management of this pathogen is crucial for ensuring rice yield and food security. In this study, we identified and characterized a novel Xoo phage, ZP3, isolated from diseased rice leaves in Zhejiang, China, which may offer new insights into biocontrol strategies against Xoo and contribute to the development of innovative approaches to combat bacterial leaf blight. Transmission electron microscopy indicated that ZP3 had a short, non-contractile tail. Genome sequencing and bioinformatic analysis showed that ZP3 had a double-stranded DNA genome with a length of 44,713 bp, a G + C content of 52.2%, and 59 predicted genes, which was similar to other OP1-type Xoo phages belonging to the genus Xipdecavirus. ZP3's endolysin LysZP was further studied for its bacteriolytic action, and the N-terminal transmembrane domain of LysZP is suggested to be a signal-arrest-release sequence that mediates the translocation of LysZP to the periplasm. Our study contributes to the understanding of phage-Xoo interactions and suggests that phage ZP3 and its endolysin LysZP could be developed into biocontrol agents against this phytopathogen.
- 相关文献
作者其他论文 更多>>
-
Risk assessment and resistant mechanism of Fusarium graminearum to fluopyram
作者：Zhang, Jie;Guo, Yu;Ren, Fuhao;Wu, Huan;Zhao, Baoquan;Duan, Yabing;Sheng, Guilin;Gu, Chunyan
关键词：Fusarium head blight; Fluopyram; Resistance risk; Resistance mechanism
-
Study on the Mechanism of Benziothiazolinone and Its Synergistic Effect with Tebuconazole in Inhibiting Fusarium asiaticum and DON Production
作者：Chen, Xing;Liu, Chuchu;Liu, Weiye;Yang, Jiawei;Sun, Yang;Cao, Haiqun;Chen, Yu;Chen, Xing;Sun, Yang;Cao, Haiqun;Chen, Yu;Chen, Xing;Sun, Yang;Cao, Haiqun;Chen, Yu;Gu, Chunyan;Cao, Haiqun;Chen, Yu
关键词：benziothiazolinone; Fusariumasiaticum; synergisticeffect; DON production
-
Effective mitigation of sclerotium rot in lettuce cultivation by two soil fungi Aspergillus terreus and Albifimbria verrucaria
作者：Li, Bing;Ijaz, Munazza;Hafeez, Rahila;Ayoade, Solabomi Olaitan;Liu, Quanhong;Zhang, Jingze;Li, Bin;Xu, Lihui;Hafeez, Rahila;Shen, Ying;Yang, Fengli;Wang, Xiao;Li, Xuqing;Gu, Chunyan
关键词：
Albifimbria verrucaria ; antagonistic fungi;Aspergillus terreus ; Butyrolactone I; Glycitein;Sclerotinia sclerotiorum -
Investigation of Antifungal Activity and Assessment of Resistance Risk of Fungicide Benzovindiflupyr in Pyricularia oryzae
作者：Sun, Yang;Liu, Xinyan;He, Ziyun;Tian, Guoqi;Liu, Weiye;Chen, Xing;Chen, Yu;Sun, Yang;Chen, Xing;Chen, Yu;Ying, Jinfeng;Gu, Chunyan;Chen, Yu
关键词：benzovindiflupyr;
Pyricularia oryzae ; succinate dehydrogenase inhibitors; resistancerisk and mechanism -
Amino acid substitutions in succinate dehydrogenase complex conferring resistance to the SDHI fungicide pydiflumetofen in Cochliobolus heterostrophus causing southern corn leaf blight
作者：Sun, Jiazhi;Yang, Bingyun;Xia, Lingmin;Yang, Rui;Ding, Chaoyang;Sun, Yang;Chen, Xing;Chen, Yu;Gu, Chunyan;Yang, Xue;Sun, Jiazhi;Yang, Bingyun;Xia, Lingmin;Yang, Rui;Ding, Chaoyang;Sun, Yang;Chen, Xing;Chen, Yu;Sun, Jiazhi;Yang, Bingyun;Xia, Lingmin;Yang, Rui;Ding, Chaoyang;Sun, Yang;Chen, Xing;Chen, Yu
关键词：Cochliobolus heterostrophus; pydiflumetofen; SDHI resistance; resistance mechanism; point mutation
-
Establishment of a system for screening and identification of novel bactericide targets in the plant pathogenic bacterium Xanthomonas oryzae pv. oryzae using Tn-seq and SPR
作者：Pang, Chaoyue;Jin, Ling;Koklannou, Damalk Saint-Claire S.;Sun, Jiazhi;Yang, Jiawei;Sun, Yang;Chen, Xing;Chen, Yu;Zang, Haoyu;Gu, Chunyan;Pang, Chaoyue;Jin, Ling;Koklannou, Damalk Saint-Claire S.;Sun, Jiazhi;Yang, Jiawei;Sun, Yang;Chen, Xing;Chen, Yu;Pang, Chaoyue;Jin, Ling;Koklannou, Damalk Saint-Claire S.;Sun, Jiazhi;Yang, Jiawei;Sun, Yang;Chen, Xing;Chen, Yu;Pang, Chaoyue;Jin, Ling;Koklannou, Damalk Saint-Claire S.;Sun, Jiazhi;Yang, Jiawei;Sun, Yang;Chen, Xing;Chen, Yu;Wang, Yongxing;Xu, Liang
关键词：rice bacterial leaf blight; Xanthomonas; essential genes; Tn-seq; dioctyldiethylenetriamine
-
Segmentation of wheat scab fungus spores based on CRF_ResUNet plus
作者：Zhang, Dongyan;Zhang, Wenhao;Cheng, Tao;Lei, Yu;Gu, Chunyan;Zhang, Dongyan;Zhang, Wenhao;Cheng, Tao;Lei, Yu;Qiao, Hongbo;Guo, Wei;Yang, Xue;Gu, Chunyan
关键词：Spore segmentation; UNet plus plus; Residual blocks; Fully connected conditional random fields; Wheat scab
