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Establishment of a system for screening and identification of novel bactericide targets in the plant pathogenic bacterium Xanthomonas oryzae pv. oryzae using Tn-seq and SPR

文献类型： 外文期刊

作者： Pang, Chaoyue 1 ; Jin, Ling 1 ; Zang, Haoyu 2 ; Koklannou, Damalk Saint-Claire S. 1 ; Sun, Jiazhi 1 ; Yang, Jiawei 1 ; Wang, Yongxing 6 ; Xu, Liang 6 ; Gu, Chunyan 2 ; Sun, Yang 1 ; Chen, Xing 1 ; Chen, Yu 1 ;

作者机构： 1.Anhui Agr Univ, Sch Plant Protect, Hefei 230036, Peoples R China

2.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei 230031, Peoples R China

3.Anhui Agr Univ, Sch Plant Protect, Anhui Prov Engn Lab Green Pesticide Dev & Applicat, Hefei 230036, Peoples R China

4.Hefei Res Ctr, State Key Lab Biol Plant Dis & Insect Pests, Hefei 230036, Peoples R China

5.Anhui Agr Univ, Sch Plant Protect, Key Lab Integrated Crop Pest Management Anhui Prov, Hefei 230036, Peoples R China

6.Shandong Vicome Greenland Chem Co Ltd, Jinan 250204, Peoples R China

关键词： rice bacterial leaf blight; Xanthomonas; essential genes; Tn-seq; dioctyldiethylenetriamine

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.6； 五年影响因子：4.5 ）

ISSN： 2095-3119

年卷期： 2024 年 23 卷 5 期

页码：

收录情况： SCI

摘要： Xanthomonas spp. cause severe bacterial diseases. However, effective strategies for prevention and management of these diseases are scarce. Thus, it is necessary to improve the efficiency of control of diseases caused by Xanthomonas. . In this study, Xanthomonas oryzae pv. oryzae (Xoo), Xoo ), which causes rice bacterial leaf blight, has been studied as a representative. A transposon insertion library of Xoo, , comprising approximately 200,000 individual insertion mutants, was generated. Transposon sequencing data indicated that the mariner C9 transposase mapped at 35.7-36.4% of all potential insertion sites, revealing 491 essential genes required for the growth of Xoo in rich media. The results show that, compared to the functions of essential genes of other bacteria, the functions of some essential genes of Xoo are unknown, 25 genes might be dangerous for the Xanthomonas group, and 3 are specific to Xanthomonas. . High-priority candidates for developing broad-spectrum, Xanthomonas-specific, and environment-friendly bactericides were identified in this study. In addition, this study revealed the possible targets of dioctyldiethylenetriamine using surface plasmon resonance (SPR) in combination with high performance liquid chromatography-mass spectrometry (HPLC-MS). The study also provided references for the research of some certain bactericides with unknown anti-bacterial mode of action. In conclusion, this study urged a better understanding of Xanthomonas, , provided meaningful data for the management of bacterial leaf blight, and disclosed selected targets of a novel bactericide.

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