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Expression of a deep-sea bacterial laccase from Halomonas alkaliantartica and its application in dyes decolorization

文献类型： 外文期刊

作者： Wang, Jingjing 1 ; Zhang, Ran 1 ; Zhu, Guilan 1 ; Wang, Lingling 1 ; Bai, Han 1 ; Qian, Yong 1 ; Zhou, Xiazhen 1 ; Yin, Qiang 2 ; Zhang, Yan 1 ;

作者机构： 1.Hefei Normal Univ, Sch Life Sci, Lianhua Rd 1688, Hefei 230601, Anhui, Peoples R China

2.Anhui Acad Agr Sci, Agr Engn Res Inst, Nongke South Rd 40, Hefei 230001, Anhui, Peoples R China

关键词： Bacterial laccase; Chloride tolerance; Dye decolorization; Redox mediator

期刊名称：ANNALS OF MICROBIOLOGY （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN： 1590-4261

年卷期： 2023 年 73 卷 1 期

页码：

收录情况： SCI

摘要： IntroductionLaccase is a copper-containing polyphenolic oxidase widely found in bacteria, archaea, fungi, animals, and plants. As a green biocatalyst with considerable potential for numerous environmental and industrial applications, the enzyme production efficiency of laccase in nature is low, and the cost is high.PurposeTo examine the characterization and potential applications of laccase in this study, a novel laccase from Halomonas alkaliantartica (LacHa) was cloned and heterologously expressed it in Escherichia coli.ResultsTo achieve heterologous and efficient laccase expression, a bacterial laccase gene designed as LacHa from Halomonas alkaliantartica of deep sea was cloned and expressed in E. coli. The results showed that the optimum temperature and pH of the enzyme reaction were 45 degrees C and 7.5. The 100 mu M Cu2+ and Fe2+ ions had the strongest stimulatory effect on laccase activity, the surface-active agent SDS and organic solvent 5% ethanol had opposite effect. EDTA, and 5% DMSO have no effect on LacHa activity.The activity of LacHa was enhanced 1.5-fold by chloride at concentrations lower than 500 mM, and 57.6% of its initial activity remained in the reaction system containing 1000 mM.NaCl. Furthermore, LacHa showed decolorization rates ranging from 90.28 to 100% for indigo carmine and two azo dyes without mediators, with wide pH (5.0-9.0) and temperature (25-65 degrees C) ranges.ConclusionsIn this study, LacHa was expressed and showed unusual properties, indicating its great application potential in textile industries or environmental fields.

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