Molecular characterization and functional analysis of the lysosomal cathepsin D-like gene in red swamp crayfish, Procambarus clarkii
文献类型： 外文期刊
作者： Huang, Long 1 ; Wu, Ben-li 1 ; He, Ji-Xiang 1 ; Zhang, Ye 1 ; Chen, Jing 1 ; Chen, Xia-jun 1 ;
作者机构： 1.Anhui Acad Agr Sci, Anhui Prov Key Lab Aquaculture & Stock Enhancemen, Fisher Inst, Hefei 230031, Peoples R China
关键词： cathepsins; crayfish; immune genes; immune system; microbial infection
期刊名称：GENOME （ 影响因子：2.166； 五年影响因子：2.474 ）
ISSN： 0831-2796
年卷期： 2021 年 64 卷 12 期
页码：
收录情况： SCI
摘要： Aspartic proteinases are one of the four families of proteinase enzymes that are widely present in living organisms. They are involved in various physiological events, such as protein degradation, development, and host defense. However, the characterization and functional roles of aspartic proteinases remain to be elucidated in crustaceans. Here, we characterized a fragment of cathepsin D-like cDNA from red swamp crayfish, Procambarus clarkii (Pc-cathepsin D-like). The open reading frame of the Pc-cathepsin D-like gene contained 1152 bp, encoding a protein of 383 amino acid residues. We also evaluated the immunological role of the Pc-cathepsin D-like gene in vivo. Spatial distribution analysis revealed that the Pc-cathepsin D-like mRNA was high in the hepatopancreas, followed by the gut, gills, and hemocytes of P. clarkii. The expression levels of the Pc-cathepsin D-like gene increased following challenge with viral (polyinosinic: polycytidylic acid) and bacterial (lipopolysaccharides, peptidoglycan) PAMPs compared with PBS injection. The suppression of the Pc-cathepsin D-like gene by RNA interference significantly increased the expression of immune-associated genes. These results showed that the Pc-cathepsin D-like gene has an essential biological role in innate immune responses because it regulates the expression of immune-associated genes.
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