文献类型： 外文期刊

作者： Xu, Xiaona ¹ ; Ping, Shuangshuang ³ ; Wang, Fen ¹ ; Chang, Muhammad Saleem ¹ ; Song, Guangtong ¹ ;

作者机构： 1.Anhui Acad Agr Sci, Fisheries Res Inst, Hefei 230031, Peoples R China

2.Key Lab Freshwater Aquaculture & Enhancement Anhui, Hefei 230031, Peoples R China

3.Yangzhou Univ, Coll Environm Sci & Engn, Yangzhou 225009, Peoples R China

4.Univ Sindh, Dept Sci & Tech Educ, Sindh 76080, Pakistan

关键词： Procambius clarkii; Folic acid; Growth; Immune response; Glucose metabolism

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：3.8 ）

ISSN： 2352-5134

年卷期： 2024 年 36 卷

页码：

收录情况： SCI

摘要： Folic acid (FA) is an essential vitamin for the growth and health of aquatic organisms in the aquaculture industry. The purpose of this study was to investigate the effects and potential mechanisms of action of dietary FA on the growth and immune response of red swamp crayfish (Procambius clarkii). Crayfish (n = 360; 3 tanks of 20 crayfish for each dietary treatment) were fed diets containing different levels of FA (0 [control], 0.5, 1, 2, 4, and 8 mg/kg dry feed weight) for 60 d. Broken-line analysis based on the final weight and specific growth rate revealed that the optimal dietary FA level for promoting growth was 2 mg/kg. Villus length, lipase activity, and antioxidant superoxide dismutase activity significantly increased under the 2 mg/kg treatment. Transcriptome analysis identified 177 upregulated and 298 downregulated differentially expressed genes (DEGs) between the 0 (control) and 2 mg/kg groups. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that the most significantly enriched pathways were related to glucose metabolism. Among the DEGs involved in glucose metabolism, Tpi1 (triosephosphate isomerase 1) and Pck1 (phosphoenolpyruvate carboxykinase 1) were upregulated and downregulated under FA treatment, respectively. Our findings suggest that the optimal dietary FA requirement for crayfish is 2 mg/kg and that dietary FA may enhance nutrient bioavailability and regulate glucose metabolism to improve crayfish growth performance.