您好，欢迎访问安徽省农业科学院 机构知识库！

Sef1, rapid-cycling Brassica napus for large-scale functional genome research in a controlled environment

文献类型： 外文期刊

作者： Xie, Xiaofang 1 ; Jiang, Yanhua 1 ; Xu, Wenqi 1 ; Yang, Weiguang 1 ; Lei, Weixia 2 ; Qian, Duo 1 ; Gao, Jie 1 ; Cai, Fangfang 1 ; Yu, Dongliang 1 ; Ke, Liping 1 ; Fan, Zhixiong 2 ;

作者机构： 1.Zhejiang Sci Tech Univ, Coll Life Sci & Med, Plant Genom & Mol Improvement Colored Fiber Lab, Hangzhou 310018, Peoples R China

2.Anhui Acad Agr Sci, Crop Inst, Hefei 230031, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2023 年 136 卷 7 期

页码：

收录情况： SCI

摘要： Key messageWe demonstrated a short-cycle B. napus line, Sef1, with a highly efficient and fast transformation system, which has great potential in large-scale functional gene analysis in a controlled environment.Rapeseed (Brassica napus L.) is an essential oil crop that accounts for a considerable share of global vegetable oil production. Nonetheless, studies on functional genes of B. napus are lagging behind due to the complicated genome and long growth cycle, this is largely due to the limited availability of gene analysis and modern genome editing-based molecular breeding. In this study, we demonstrated a short-cycle semi-winter-type Brassica napus 'Sef1' with very early-flowering and dwarf phenotype, which has great potential in large-scale indoor planting. Through the construction of an F-2 population of Sef1 and Zhongshuang11, bulked segregant analysis (BSA) combined with the rape Bnapus50K SNP chip assay method was used to identify the early-flowering genes in Sef1, and a mutation in BnaFT.A02 was identified as a major locus significantly affecting the flowering time in Sef1. To further investigate the mechanism of early flowering in Sef1 and discover its potential in gene function analysis, an efficient Agrobacterium-mediated transformation system was established. The average transformation efficiency with explants of hypocotyls and cotyledons was 20.37% and 12.8%, respectively, and the entire transformation process took approximately 3 months from explant preparation to seed harvest of transformed plants. This study demonstrates the great potential of Sef1 for large-scale functional gene analysis.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台