文献类型： 外文期刊

作者： Wu, Wangze ¹ ; Wu, Yujun ¹ ; Gao, Yang ¹ ; Li, Meizhen ¹ ; Yin, Hongju ¹ ; Lv, Minghui ¹ ; Zhao, Jianxin ¹ ; Li, Jia ¹ ; He, ¹ ;

作者机构： 1.Lanzhou Univ, Minist Educ, Sch Life Sci, Key Lab Cell Activ & Stress Adaptat, Lanzhou 730000, Peoples R China

2.Anhui Acad Agr Sci, Crop

关键词： Arabidopsis;brassinosteroid;cell death;LRR-RLK;natural variation;RD kinase;SERK;signal transduction

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2015 年 6 卷

页码：

收录情况： SCI

摘要： In plants, LRR-RLKs play central roles in regulating perception of extracellular signals and initiation of cellular responses under various environmental challenges. Arabidopsis SERK genes, including SERK1 to SERK5, constitute a LEE-ELK sub-family. SERK1, SERK2, SERK3/BAK1. and SERK4/BKK1 have been well characterized to function as crucial regulators in multiple physiological processes such as brassinosteroid signaling, cell death control, pathogenesis, and pollen development. Despite extremely high sequence identity with BKK1, SERK5 is reported to have no functional overlapping with BKK1, which is previously identified to regulate BR and cell death control pathways, probably due to a natural mutation in a highly conserved RD motif in the kinase domain of SERK5 in Col-0 ecotype. Through a gene sequencing analysis in several Arabidopsis accessions, we are able to identify SERK5 in Landsberg erecta (Ler) genome encoding a LRR-RLK with an intact RD motif. Overexpression of SERK5-Ler partially suppresses the BR defective phenotypes of bril -5 and bakl -3 bkkl -7, indicating SERK5-Ler functions as a positive regulator in BR signaling. Furthermore, the interaction between SERK5-Ler and BRI1 is confirmed by yeast two-hybrid and BiFC assays, and the genetic result showing that elevated expression of a kinase-dead form of SERK5-Ler causes a dominant-negative phenotype in bril -5. In addition, overexpression of SERK5-Ler is capable of delaying, not completely suppressing, the cell death phenotype of bakl -3 bkk1 -1. In this study, we first reveal that SERK5-Ler is a biologically functional component in mediating multiple signaling pathways.