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Identification and expression profiles of chitin deacetylase genes in the rice leaf folder, Cnaphalocrocis medinalis

文献类型： 外文期刊

作者： Yu, Hai-Zhong 1 ; Liu, Ming-Hui 2 ; Wang, Xue-Yang 1 ; Yang, Xin 1 ; Wang, Wan-Ling 3 ; Geng, Lei 1 ; Yu, Dong 1 ; Liu, X 1 ;

作者机构： 1.Anhui Agr Univ, Sch Life Sci, 130 West Changjiang Rd, Hefei 230036, Peoples R China

2.Anhui Acad Agr Sci, Inst Sericulture, 15 Huoshan Rd, Hefei 230061, Peoples R China

3.Anhui Acad Agr Sci, Inst Rice, 40 South Nongke Rd, Hefei 230031, Peoples R China

关键词： Cnaphalocrocis medinalis;Chitin deacetylase (CDA);Developmental and tissue-specific expression

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）

ISSN： 1226-8615

年卷期： 2016 年 19 卷 3 期

页码：

收录情况： SCI

摘要： Chitin deacetylase (CDA) is an insect chitin degradation enzyme that catalyzes the deacetylation of chitin to form chitosan. In this study, combination of rapid-amplification of cDNA ends (RACE) technology with Cnaphalocrocis medinalis transcriptome database analysis revealed the presence of at least five C. medinalis CDAs (CmCDAs), which were CmCDA1, CmCDA2, CmCDA4, CmCDA5, and CmCDA6. The cDNA sequences of CmCDA1, CmCDA2, and CmCDA4 had whole open reading frame (ORE) for further analysis. Phylogenetic analysis indicated that insect CDAs could be categorized into five groups. CmCDAs' structural domain analysis revealed that all three CDAs contained the chitin deacetylase-like catalytic domain. CmCDA1 and CmCDA2 belong to Group I because they both contain the chitin-binding peritrophin-A domain (ChBD), low-density lipoprotein receptor class A domain (LDLa), and chitin deacetylase-like catalytic domain. CmCDA4 only contains ChBD and chitin deacetylase-like catalytic domain thus belongs to Group III. Tissue and developmental stage expression analysis showed that the expression levels of CmCDA1, CmCDA2, and CmCDA4 are significantly higher in the head than other tissues and also significantly higher in adults than in larvae. CmCDA5 had significantly higher expression in the integument than other tissues, suggesting potential roles in the process of degradation of chitin. In contrast CmCDA5 showed relatively high expression in larvae. In conclusion, this study analyzed the cDNA sequences of three CDA genes and determined their expression and molecular characteristics, which provided a new sequence resource and improved the development of bio-pesticides and the biological pest control and contributed to management of this important agricultural pest. (C) 2016 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.

  • 相关文献

[1]Identification of Genes Putatively Involved in Chitin Metabolism and Insecticide Detoxification in the Rice Leaf Folder (Cnaphalocrocis medinalis) Larvae through Transcriptomic Analysis. Yu, Hai-Zhong,Wen, De-Fu,Geng, Lei,Xu, Jia-Ping,Wang, Wan-Lin,Zhang, Yan. 2015

[2]Identification of Four ATP-Binding Cassette Transporter Genes in Cnaphalocrocis medinalis and Their Expression in Response to Insecticide Treatment. Yu, Hai-Zhong,Xu, Jia-Ping,Wang, Xue-Yang,Ma, Yan,Yu, Dong,Fei, Dong-Qiong,Zhang, Shang-Zhi,Wang, Wan-Ling. 2017

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