您好，欢迎访问安徽省农业科学院 机构知识库！

Immune Functional Analysis of Chitin Deacetylase 3 from the Asian Citrus Psyllid Diaphorina citri

文献类型： 外文期刊

作者： Yu, Hai-Zhong 1 ; Li, Ning-Yan 1 ; Li, Bing 3 ; Toufeeq, Shahzad 4 ; Xie, Yan-Xin 1 ; Huang, Yu-Ling 1 ; Du, Yi-Ming 1 ;

作者机构： 1.Gannan Normal Univ, Sch Life Sci, Ganzhou 341000, Peoples R China

2.Gannan Normal Univ, Natl Navel Orange Engn & Technol Res Ctr, Ganzhou 341000, Peoples R China

3.Anhui Acad Agr Sci, Inst Sericulture, Hefei 230031, Peoples R China

4.Anhui Agr Univ, Sch Life Sci, Hefei 230031, Peoples R China

关键词： Diaphorina citri; chitin deacetylase 3; expression profiles; immune response

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Chitin deacetylase (CDA) is a chitin degradation enzyme that strictly catalyzes the deacetylation of chitin to form chitosan, which plays an important role in regulating growth and development, as well as the immune response. In this study, a chitin deacetylase 3 gene (CDA3) was identified with a complete open reading frame (ORF) of 1362 bp from the genome database of Diaphorina citri, encoding a protein of 453 amino acids. Spatiotemporal expression analysis suggested that D. citriCDA3 (DcCDA3) had the highest expression level in the integument and third-instar nymph stage. Furthermore, DcCDA3 expression level can be induced by 20-hydroxyecdysone (20E). Injection of Escherichia coli and Staphylococcus aureus induced the upregulation of DcCDA3 in the midgut, while DcCDA3 was downregulated in the fat body. After silencing DcCDA3 by RNA interference, there was no influence on the D. citri phenotype. In addition, bactericidal tests showed that recombinant DcCDA3 inhibited gram-positive bacteria, including S. aureus and Bacillus subtilis (B. subtilis). In conclusion, our results suggest that DcCDA3 might play an important role in the immune response of D. citri.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台