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Discrimination of transgenic soybean seeds by terahertz spectroscopy

文献类型: 外文期刊

作者: Liu, Wei 1 ; Liu, Changhong 1 ; Chen, Feng 3 ; Yang, Jianbo 4 ; Zheng, Lei 1 ;

作者机构: 1.Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China

2.Hefei Univ, Intelligent Control & Compute Vis Lab, Hefei 230601, Peoples R China

3.Clemson Univ, Dept Food Nutr & Packaging Sci, Clemson, SC 29634 USA

4.Anhui Acad Agr Sci, Rice Res Inst, Hefei 230031, Peoples R China

5.Hefei Univ Technol, Sch Med Engn, Hefei 230009, Peoples R China

期刊名称:SCIENTIFIC REPORTS ( 影响因子:4.379; 五年影响因子:5.133 )

ISSN: 2045-2322

年卷期: 2016 年 6 卷

页码:

收录情况: SCI

摘要: Discrimination of genetically modified organisms is increasingly demanded by legislation and consumers worldwide. The feasibility of a non-destructive discrimination of glyphosate-resistant and conventional soybean seeds and their hybrid descendants was examined by terahertz time-domain spectroscopy system combined with chemometrics. Principal component analysis (PCA), least squares-support vector machines (LS-SVM) and PCA-back propagation neural network (PCA-BPNN) models with the first and second derivative and standard normal variate (SNV) transformation pre-treatments were applied to classify soybean seeds based on genotype. Results demonstrated clear differences among glyphosate-resistant, hybrid descendants and conventional non-transformed soybean seeds could easily be visualized with an excellent classification (accuracy was 88.33% in validation set) using the LS-SVM and the spectra with SNV pre-treatment. The results indicated that THz spectroscopy techniques together with chemometrics would be a promising technique to distinguish transgenic soybean seeds from non-transformed seeds with high efficiency and without any major sample preparation.

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