安徽省农业科学院机构知识库

Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit

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文献类型：外文期刊

作者： Qin Gai-hua ¹ ; Qi Xiao-xiao ² ; Qi Yong-jie ¹ ; Gao Zheng-hui ¹ ; Pan Hai-fa ¹ ; Xu Yi-liu ¹ ;

作者机构： 1.Anhui Acad Agr Sci, Inst Hort Res, Hefei 230031, Anhui, Peoples R China

2.Anhui Key Lab Genet Improvement & Ecophysiol Hort, Hefei 230031, Anhui, Peoples R China

3.Anhui Radio & Televis Univ, Coll Agr Med & Hlth, Hefei 230022, Anhui, Peoples R China

关键词： alcohol dehydrogenase;ester volatile;gene expression;pear

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （影响因子：2.848；五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2017 年 16 卷 8 期

页码：

收录情况： SCI

摘要： Alcohol dehydrogenase (ADH) catalyzes the interconversion of aldehydes and their corresponding alcohols, and is a key enzyme in volatile ester biosynthesis. However, little is known regarding ADH and ADH encoding genes (ADHs) in pear. We identified 8 ADHs in the pear's genome (PbrADHs) by multiple sequences alignment. The PbrADHs were highly homologous in their coding regions, while were diversiform in structure. 9 introns were predicted in PbrADH(3)-PbrADH(8), while 8 introns, generated through exon fusion and intron loss, were predicted in PbrADH, and PbrADH(2). To study the genetic regulation underlying aroma biogenesis in pear fruit, we determined the PbrADH transcripts, ADH activities and volatile contents of fruits during ripening stage for Nanguoli and Dangshansuli, two cultivars having different aroma characteristics. ADH activity was strongly associated with the transcription of ADH(6) in the two cultivars during fruit ripening stage. The higher ester content paralleling to a higher ADH activity was detected in Nanguoli than in Dangshansuli, so it is induced that the lower ester content in Dangshansuli fruit may be the result of weak ADH activity. The present study revealed that total ADH activity and volatile ester production correlated with increased PbrADH(6) transcript levels. PbrADH(6) may contribute to ADH activity catalyzing aldehyde reduction and ester formation in pear fruit.

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Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit

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Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit

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