The Bacteria-Derived dsRNA Was Used for Spray-Induced Gene Silencing for Rice False Smut Control
文献类型： 外文期刊
作者： Zhang, Yujie 1 ; Wu, Mengyao 1 ; Zhang, Han 1 ; Li, Yu 1 ; Wang, Yufu 1 ; Meng, Fanzhu 1 ; Zhao, Wei 2 ; He, Shun 3 ; Yin, Weixiao 1 ; Luo, Chao-Xi 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Key Lab Plant Pathol, Wuhan 430070, Peoples R China
2.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei 230001, Peoples R China
3.Huazhong Agr Univ, Coll Plant Sci & Technol, Ctr Crop Nanobiotechnol, Wuhan 430070, Peoples R China
关键词： Ustilaginoidea virens; rice false smut; double-strandedRNA; in vivo; spray-induced gene silencing; RNA interference; disease management
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 51 期
页码：
收录情况： SCI
摘要： False smut caused by Ustilaginoidea virens is one of the most destructive diseases in rice. The disease is primarily controlled with fungicides, leading to the development of fungicide resistance. Although spray-induced gene silencing (SIGS) has been utilized for disease management, it has not been applied to control rice false smut. In this study, we introduce a novel approach involving the in vivo synthesis and exogenous application of double-stranded RNA (dsRNA) to manage rice false smut disease. The UvCYP51, UvBI-1, and UvbZIP11 genes were selected as target genes and highly efficient fragments for gene silencing were identified through screening of silencing transformants. Although direct dsRNA uptake by U. virens was not observed, in vivo synthesis and application of dsRNA to rice effectively reduced the expression of target genes. Treatment with dsRNA targeting the genes resulted in a decrease in smut balls, providing a promising disease management strategy against rice false smut.
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