The receptor-like protein RXEG1 confers broad-spectrum resistance in soybean and cotton by recognizing microbial glycoside hydrolase 12 proteins
文献类型： 外文期刊
作者： Zeng, Mengzhu 1 ; Qiu, Ping 4 ; Ouyang, Haibing 1 ; Sun, Yujing 1 ; Chen, Zhiyuan 1 ; Cheng, Yuanyuan 1 ; Zhang, Mingmei 1 ; Ye, Yu 1 ; Wang, Lei 1 ; Zheng, Zuling 1 ; Wang, Hao 1 ; Liu, Tianyao 1 ; Zhao, Wei 6 ; Duan, Kaixuan 1 ; Wang, Yan 1 ; Zhu, Longfu 4 ; Wang, Yuanchao 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, State Key Lab Agr & Forestry Biosecur, Nanjing, Peoples R China
2.Nanjing Agr Univ, Zhongshan Biol Breeding Lab ZSBBL, Nanjing, Peoples R China
3.Nanjing Agr Univ, Key Lab Soybean Dis & Pest Control, Minist Agr & Rural Affairs, Nanjing, Peoples R China
4.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China
5.Hunan Agr Univ, Coll Agron, Changsha, Peoples R China
6.Anhui Acad Agr Sci, Inst Plant Protect & Agro Prod Safety, Hefei, Peoples R China
关键词：
plant immune receptor; immune recognition; glycoside hydrolase 12 proteins; soybean disease;
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.5； 五年影响因子：12.4 ）
ISSN： 1467-7644
年卷期： 2025 年 23 卷 8 期
页码：
收录情况： SCI
摘要： Plants diseases cause substantial crop yield loss and threaten food security. Enhancing plant genetic resistance is a major strategy to mitigate the impact of plant diseases on agricultural production. The leucine-rich repeat receptor-like protein RXEG1 recognizes the glycoside hydrolase 12 (GH12) protein XEG1 secreted by Phytophthora sojae to mount immune responses in Nicotiana benthamiana. Here, we found that RXEG1 broadly recognizes multiple GH12 proteins of oomycete and fungal pathogens. Structural modelling and mutagenesis analyses of critical interacting residues revealed that RXEG1 binds to the enzyme-activated pocket of different GH12 proteins through an amino-terminal and a carboxy-terminal loopout region (RXEG1ID), forming a conserved interface with various GH12 proteins. Furthermore, the binding of RXEG1 to the active-site groove of GH12 proteins inhibits their hydrolase activity. Heterologous expression of RXEG1 in soybean and cotton confers enhanced resistance against a wide range of oomycete and fungal pathogens without growth penalties. Our data reveal that leveraging the conserved recognition and inhibition functions towards GH12 proteins enables RXEG1 application in crops to enhance resilience against various economically significant pathogens.
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