您好，欢迎访问安徽省农业科学院 机构知识库！

Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars

文献类型： 外文期刊

作者： Ding, Haisheng 1 ; Liu, Min 1 ; Zhou, Changfan 1 ; You, Xiangbin 1 ; Su, Tao 1 ; Yang, Youbing 4 ; Xu, Dequan 1 ;

作者机构： 1.Huazhong Agr Univ, Key Lab Swine Genet & Breeding, Minist Agr & Rural Affairs, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China

3.Anhui Acad Agr Sci, Anhui Key Lab Livestock & Poultry Prod Safety, Inst Anim Husb & Vet Med, Hefei 230031, Peoples R China

4.Henan Univ Sci & Technol, Coll Anim Sci & Technol, Luoyang 471023, Peoples R China

关键词： Meishan boar; Duroc boar; Testis; Sexual development; miRNA; Integrating analysis

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundMicroRNAs (miRNAs) are small non-coding RNAs playing vital roles in regulating posttranscriptional gene expression. Elucidating the expression regulation of miRNAs underlying pig testis development will contribute to a better understanding of boar fertility and spermatogenesis.ResultsIn this study, miRNA expression profile was investigated in testes of Duroc and Meishan boars at 20, 75, and 270days of age by high-throughput sequencing. Forty-five differentially expressed miRNAs were identified from testes of Duroc and Meishan boars before and after puberty. Integrated analysis of miRNA and mRNA profiles predicted many miRNA-mRNA pairs. Gene ontology and biological pathway analyses revealed that predicted target genes of ssc-mir-423-5p, ssc-mir-34c, ssc-mir-107, ssc-mir-196b-5p, ssc-mir-92a, ssc-mir-320, ssc-mir-10a-5p, and ssc-mir-181b were involved in sexual reproduction, male gamete generation, and spermatogenesis, and GnRH, Wnt, and MAPK signaling pathway. Four significantly differentially expressed miRNAs and their predicted target genes were validated by quantitative real-time polymerase chain reaction, and phospholipase C beta 1 (PLC beta 1) gene was verified to be a target of ssc-mir-423-5p.ConclusionsThis study provides an insight into the functional roles of miRNAs in testis development and spermatogenesis and offers useful resources for understanding differences in sexual function development caused by the change in miRNAs expression between Duroc and Meishan boars.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台