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Comparative Analysis of Angora Rabbit Colostrum and Mature Milk Using Quantitative Proteomics

文献类型： 外文期刊

作者： Huang, Dongwei 1 ; Wang, Yuanlang 1 ; Ding, Haisheng 1 ; Zhao, Huiling 1 ;

作者机构： 1.Anhui Acad Agr Sci, Inst Anim Husb & Vet Med, Anhui Prov Key Lab Livestock & Poultry Prod Safety, Hefei 230031, Peoples R China

关键词： rabbit milk; colostrum; mature milk; proteomic; DIA

期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）

ISSN：

年卷期： 2024 年 13 卷 8 期

页码：

收录情况： SCI

摘要： Simple Summary Rabbit colostrum is the initial mammary secretion after parturition, consisting of nutritional and bioactive components. However, the protein expression profiles in the colostrum and mature milk of rabbits are still unclear. Accordingly, in this study, the proteomics approach was performed on milk sampled from rabbit colostrum and mature milk. A total of 525 differentially abundant proteins (DAPs) were revealed, among which 244 proteins were upregulated and 281 were downregulated compared with the colostrum of rabbits. The bioinformatics analysis showed that the DAPs were related to the immune response and fatty acids metabolism. In conclusion, the results of this study will contribute to the expansion of the rabbit milk protein database, as well as provide insights into utilizing breeding practices to address concerns related to the growth and health of rabbit offspring.Abstract Colostrum intake is a crucial determinant of survival in newborn rabbits. Neonates rely entirely on passive immunity transfer from their mothers while suckling colostrum. The goal of this study was to explore the protein differences of rabbit milk during different lactation periods. Our findings showed that the daily milk yield exhibited an increasing trend from the 2nd to the 21st day of lactation. A data-independent acquisition proteomics approach identified a total of 2011 proteins. Significantly, different abundances were found for 525 proteins in the colostrum and the mature milk samples. Eleven differentially abundant proteins (DAPs) were examined using parallel reaction monitoring, which verified the reliability of the proteomic data. Gene Ontology analysis revealed that these DAPs were primarily associated with glycosyltransferase activity, macromolecule transmembrane transporter activity, and regulation of acute inflammatory response. The dominant metabolic pathways of the DAPs involve the complement and coagulation cascades. A protein-protein interaction analysis identified apolipoprotein B, apolipoprotein A1, triose phosphate isomerase 1, and albumin as the hub proteins responsible for distinguishing differences between biological properties in rabbit colostrum and mature milk. These findings enhance our comprehension of the rabbit milk proteome, particularly in expanding our knowledge regarding the requirements of neonatal rabbits.

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