文献类型： 外文期刊

作者： Zhu, H. L. ¹ ; Zhao, X. W. ¹ ; Wang, X. Z. ² ; Qi, Y. X. ¹ ; Huang, D. W. ¹ ; Cheng, G. L. ¹ ; Zhao, H. L. ¹ ; Yang, Y. X ¹ ;

作者机构： 1.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Anhui Prov Key Lab Livestock & Poultry Prod Safet, Hefei 230031, Peoples R China

2.Qinghai Prov Ctr Dis Control & Prevent, Xining 810010, Peoples R China

关键词： Fc receptor; antimicrobial peptide; small intestine; neonatal calf

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）

ISSN： 0022-0302

年卷期： 2020 年 103 卷 10 期

页码：

收录情况： SCI

摘要： The contribution of colostrum to passive immunity transfer and intestinal protection is well known; however, the effects of colostrum intake on the expression of antimicrobial peptides (AP) and Fc receptors in the intestine of neonatal calves are unclear. Our aim was to investigate changes in the expression of AP and Fc receptor in the small intestine of calves in the first 36 II postpartum. Twenty-four Holstein bull calves were used in this study, of which 18 calves were administered 3.2 L of pooled colostrum for each calf per meal via an esophageal tube. Calves were slaughtered at 8 h (1 meal at 1-2 h), 24 h (2 meals at 1-2 h and 10-12 h), and 36 h (3 meals at 1-2 h, 10-12 h, and 22-24 h) postpartum. The remaining 6 calves without any milk administration were slaughtered at 2 h postpartum. Samples of blood and jejunum digesta were collected to determine immunoglobulin concentration using ELISA. Samples of the duodenum, jejunum, and ileum tissues after slaughter were collected to determine AP and Fc receptor expression using quantitative real-time PCR. In calves administered colostrum, IgG concentration in jejunum digesta rapidly decreased in an age-dependent manner (33.41, 9.47, and 0.34 mg/mL at 8, 24, and 36 h, respectively), whereas serum IgG concentration increased significantly, from 0.25 mu g/mL at 2 h to 21.72 mg/mL at. 24 h. Cathelicidin-4, beta-defensin (DEFB)-7, and enteric beta-defensin expression was upregulated at 8 h postpartum in the duodenum and jejunum compared with that at 2 h, but progressive recovery was detected from 24 h onward. Higher expression of cathelicidin-4, regenerating family member 3 gamma, lysozyme (LYZ), LYZ1, and LYZ2 and lower expression of DEFB, DEPB1, DEFB7, DEFB10, and enteric beta-defensin were observed in the duodenum and jejunum compared with the ileum. Differences in AP expression between intestinal regions suggested that the innate immune defense mechanism varied significantly among the duodenum, jejunum, and ileum. No difference in the expression of Fc fragment of the IgG receptor was observed either among ages or small intestinal regions. The Fc gamma receptor (Fc gamma R)Ia and Fc-gamma RIIb expression was the highest at 8 h compared with that at 2, 24, and 36 h, and expression of Fc gamma RIa, Fc gamma RIIb, and Fc gamma RIlla was higher in the duodenum and jejunum than in the ileum. These results indicated that AP and Fc gamma receptors might play important roles in intestinal defense during the passive immunity period.