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Bacterial laccase ofAnoxybacillus ayderensisSK3-4 from hot springs showing potential for industrial dye decolorization

文献类型： 外文期刊

作者： Wang, Jingjing 1 ; Chang, Fei 2 ; Tang, Xiaoqing 3 ; Li, Wei 1 ; Yin, Qiang 4 ; Yang, Yang 2 ; Hu, Yang 1 ;

作者机构： 1.Hefei Normal Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China

2.Hefei Univ, Sch Biol Food & Environm, Hefei 230601, Anhui, Peoples R China

3.Florida Int Univ, Dept Chem & Biochem, Miami, FL 33199 USA

4.Anhui Acad Agr Sci, Agr Engn Res Inst, Hefei 230031, Anhui, Peoples R China

关键词： Anoxybacillus ayderensisSK3-4; Laccase; Purification; Characterization; Decolorization

期刊名称：ANNALS OF MICROBIOLOGY （ 影响因子：2.112； 五年影响因子：2.151 ）

ISSN： 1590-4261

年卷期： 2020 年 70 卷 1 期

页码：

收录情况： SCI

摘要： Introduction Laccases are green biocatalysts that possess attractive for the treatment of resistant environmental pollutants and dye effluents. Purpose To exploit the laccase ofAnoxybacillus ayderensisSK3-4 that possesses dye decolorization ability at room and higher temperature, we characterized the enzyme in considerable detail and investigated its ability to decolorize different dyes. Results A bacterial laccase gene designed asLacAnfromAnoxybacillus ayderensisSK3-4 of hot springs was cloned and expressed inEscherichia coli. LacAn is a monomeric protein with a molecular weight of 29.8 kDa. The optimum pH and temperature for syringaldazine oxidation were 7.0 and 75 degrees C, respectively. LacAn was stable at pH values ranging from 6.5 to 8.5 above 65 degrees C. The enzyme activity was significantly enhanced by Cu(2+)and Mg(2+)but inhibited by Zn(2+)and Fe2+. Furthermore, LacAn showed high decolorization capability toward five dyes (direct blue 6, acid black 1, direct green 6, direct black 19, and acid blue 93) in the absence of redox mediators. It also demonstrated a wide temperature range, and it can retain its high decolorization ability even at high temperatures. Conclusions These properties including better enzymatic properties and efficiency to decolorize dyes demonstrate that the bacterial laccase LacAn has potentials for further industrial applications.

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