Bombyx mori Serpin6 regulates prophenoloxidase activity and the expression of antimicrobial proteins
文献类型： 外文期刊
作者： Li, Bing 1 ; Yu, Hai-Zhong 1 ; Ye, Chong-Jun 1 ; Ma, Yan 1 ; Li, Xing 2 ; Fan, Tao 2 ; Chen, Fu-Sheng 2 ; Xu, Jia-Ping 1 ;
作者机构： 1.Anhui Agr Univ, Sch Iife Sci, 130 West Changjiang Rd, Hefei 230036, Peoples R China
2.Anhui Acad Agr Sci, Inst Sericulture, 15 Huoshan Rd, Hefei 230061, Peoples R China
关键词： Serpin6;Prophenoloxidase;Antimicrobial peptides;Bombyx mori
期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Serpins are a family of serine protease inhibitors that are found widely in insects. They play an important role in insect physiological responses, such as innate immunity and development. In this study, we obtained the Bombyx mori serpin6 (BmSerpin6) sequence from National Center for Biotechnology Information (NCBI) and the silkworm genome database (SilkDB). Reverse transcription PCR (RT-PCR) results showed that BmSerpin6 was expressed highly in hemocytes, the midgut, and the fat body. After challenging with Micrococcus luteus (Mi) and Serratia marcescens (Sm), the BmSerpin6 expression level was induced significantly. Transcript levels of gloverin2 and prophenoloxidase (PPO) activity were reduced significantly in the fat body and hemocytes after injecting the recombinant BmSerpin6 protein into silkworm larvae. A BmSerpin6 recombinant plasmid (BmSerpin6-pAC 5.1) was constructed successfully and transfected into Drosophila S2 cells, which resulted in significantly reduced expression of the drosomycin protein. These results indicated that BmSerpin6 might regulate silkworm immune responses. (C) 2017 Elsevier B.V. All rights reserved.
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