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Effect of chicken leptin recptor short hairpin RNA on expression of JAK2, STAT3, SOCS3 and CPT1 genes in chicken preadipocytes

文献类型： 外文期刊

作者： Ban, Qian 1 ; Hui, Wenqiao 2 ; Cheng, Fei 1 ; Liu, Dahai 1 ; Liu, Xiaojun 3 ;

作者机构： 1.Anhui Univ, Sch Life Sci, Rd Jiulong, Hefei 230601, Anhui, Peoples R China

2.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Hefei, Peoples R China

3.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China

4.Shihezi Univ, Coll Anim Sci & Technol, Rd Beisi, Shihezi 832003, Xinjiang, Peoples R China

关键词： chicken;LEPR;preadipocytes;RNAi

期刊名称：ANIMAL SCIENCE JOURNAL （ 影响因子：1.749； 五年影响因子：1.909 ）

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收录情况： SCI

摘要： In this study, we detect depressive effect on leptin receptor (LEPR) by LEPR-specific short hairpin RNA (shRNA) expression plasmids in chicken preadipocytes, and effect on messenger RNA (mRNA) expression levels of genes related to signal transduction, including JAK2, STAT3, SOCS3 as well as CPT1, which is associated with fatty acid metabolism. shRNA expression vectors targeting LEPR were constructed and transfected into chicken preadipocytes. The transfection efficiency was evaluated by fluorescence microscopy. Real-time PCR was used to detect its effect on mRNA expression levels of JAK2, STAT3, SOCS3 and CPT1. Results showed that LEPR mRNA was knocked down by 99% (P<0.01) after transfection for 72h. In the knockdown preadipocytes, the mRNA levels of JAK2 and CPT1 were down-regulated by 47.56% (P<0.01) and 42.26% (P<0.05), respectively; while expression of STAT3 and SOCS3 increased 7.72-fold (P<0.01), 1.71-fold (P<0.01), respectively. It is concluded that knockdown of LEPR influences mRNA expression of its down-stream genes, suggesting that chicken LEPR play a certain role in regulating genes in the complicated gene network of preadipocytes.

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