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Determination of changes in bovine plasma and milk proteins during naturally occurring Escherichia coli mastitis by comparative proteomic analysis

文献类型： 外文期刊

作者： Yang, Yongxin 1 ; Cao, Suizhong 2 ; Zhao, Xiaowei 1 ; Huang, Dongwei 1 ; Zhao, Huiling 1 ; Cheng, Guanglong 1 ;

作者机构： 1.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Hefei 230031, Peoples R China

2.Sichuan Agr Univ, Coll Vet Med, Yaan 625014, Peoples R China

3.Michigan State Univ, Coll Vet Med, E Lansing, MI 48824 USA

关键词： dairy cows;proteome

期刊名称：ANIMAL PRODUCTION SCIENCE （ 影响因子：1.533； 五年影响因子：1.779 ）

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收录情况： SCI

摘要： The aim of this study was to investigate changes in plasma and milk proteins in response to Escherichia coli infection in cows. Plasma and milk were collected from healthy cows, cows suffering from mild E. coli mastitis, and cows suffering from severe E. coli mastitis. Protein composition was examined by two-dimensional gel electrophoresis coupled with mass spectrometry. Plasma haptoglobin and alpha-1 acid glycoprotein demonstrated greater expression in mastitic cows compared with controls, but there were no difference between mildly and severely mastitic cows. Milk from mildly mastitic cows showed increased albumin and casein variants. Severely mastitic cows showed lower casein levels and increased anti-microbial and acute phase proteins. Milk alpha-1 acid glycoprotein and cathelicidins were associated with severe mastitis. A greater number of beta-casein fragments that corresponded to beta-casein isoforms were found in milk from mildly mastitic cows. These results suggest that caseins levels decreased and the concentrations of anti-microbial and acute phase proteins increased corresponding to the degree of E. coli mastitis. Nevertheless, further studies are needed to determine whether cathelicidin could serve as a diagnostic marker for mastitis.

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[2]Comparative Proteomic Analysis of Plasma from Clinical Healthy Cows and Mastitic Cows. Yang Yong-xin,Zhao Xing-xu,Zhang Yong,Yang Yong-xin. 2009

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