文献类型： 外文期刊
作者： Zhu, Qili 1 ; Zhou, Benguo 2 ; Gao, Zhengliang 2 ; Liang, Yuancun 1 ;
作者机构： 1.Shandong Agr Univ, Dept Plant Pathol, Tai An 271018, Shandong, Peoples R China
2.Anhui Acad Agr Sci, Tobacco Res Inst, Hefei 230031, Peoples R China
期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）
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收录情况： SCI
摘要： Phospholipase C (PLC) plays important roles in regulating various biological processes in eukaryotes. Currently, little is known about the function of PLC in filamentous fungi, especially the plant pathogenic fungi. Fusarium graminearum is the causal agent of Fusarium head blight in many cereal crops. BLAST search revealed that Fusarium genome contains six FgPLC genes. Using quantitative RT-PCR, different FgPLC gene expressions in mycelia were analyzed. To investigate the role of FgPLC in F. graminearum biology, a pharmacological study using a known inhibitor of PLC (U73122) was conducted. Results showed that inhibition of FgPLC resulted in significant alterations of mycelial growth, conidiation, conidial germination, perithecium formation, and expressions of Tri5 and Tri6 genes. As expected, the treatment of F. graminearum with U73343, an inactive analog of U73122, showed no effect on F. graminearum biology. Our results suggested strongly that FgPLC plays important roles in F. graminearum growth and development.
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