Hydrogen peroxide production and mitochondrial dysfunction contribute to the fusaric acid-induced programmed cell death in tobacco cells
文献类型： 外文期刊
作者： Jiao, Jiao 1 ; Sun, Ling 1 ; Zhou, Benguo 2 ; Gao, Zhengliang 2 ; Hao, Yu 1 ; Zhu, Xiaoping 1 ; Liang, Yuancun 1 ;
作者机构： 1.Shandong Agr Univ, Dept Plant Pathol, Tai An 271018, Shandong, Peoples R China
2.Anhui Acad Agr Sci, Tobacco Res Inst, Hefei 230031, Anhui, Peoples R China
关键词： Fusaric acid;Programmed cell death;Hydrogen peroxide;Mitochondrion;Tobacco suspension cells
期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.549； 五年影响因子：4.164 ）
ISSN： 0176-1617
年卷期： 2014 年 171 卷 13 期
页码：
收录情况： SCI
摘要： Fusaric acid (FA), a non-specific toxin produced mainly by Fusarium spp., can cause programmed cell death (PCD) in tobacco suspension cells. The mechanism underlying the FA-induced PCD was not well understood. In this study, we analyzed the roles of hydrogen peroxide (H2O2) and mitochondrial function in the FA-induced PCD. Tobacco suspension cells were treated with 100 mu M FA and then analyzed for H2O2 accumulation and mitochondrial functions. Here we demonstrate that cells undergoing FA-induced PCD exhibited H2O2 production, lipid peroxidation, and a decrease of the catalase and ascorbate peroxidase activities. Pre-treatment of tobacco suspension cells with antioxidant ascorbic acid and NADPH oxidase inhibitor diphenyl iodonium significantly reduced the rate of FA-induced cell death as well as the caspase-3-like protease activity. Moreover, FA treatment of tobacco cells decreased the mitochondrial membrane potential and ATP content. Oligomycin and cyclosporine A, inhibitors of the mitochondrial ATP synthase and the mitochondrial permeability transition pore, respectively, could also reduce the rate of FA-induced cell death significantly. Taken together, the results presented in this paper demonstrate that H2O2 accumulation and mitochondrial dysfunction are the crucial events during the FA-induced PCD in tobacco suspension cells. (C) 2014 Elsevier GmbH. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Identification and complete genome sequence of iris potyvirus A, which causes dwarfing and foliar chlorosis with mosaic or mottle disease symptoms on lily (Lilium lancifolium Thunb.) in China
作者：Wang, Fang;Yan, Dankan;Han, Kelei;Ma, Chao;Chen, Ying;Gao, Zhengliang;Bao, Xianxun;Li, Cheng
关键词：
-
Development of Recombinase Polymerase Amplification Combined with Lateral Flow Detection Assay for Rapid and Visual Detection of Ralstonia solanacearum in Tobacco
作者：Li, Changfeng;Yan, Xiaoming;Ju, Yuliang;Shen, Pengfei;Wu, Xun;Cao, Le;Pan, Yuemin;Zhou, Benguo
关键词：Ralstonia solanacearum; recombinase polymerase amplification; molecular diagnosis; specificity; sensitivity
-
Characterisation of a novel alphaendornavirus isolated from balloon flower (Platycodon grandiflorus)
作者：Wang, Fang;Dong, Qing;Gao, Zhengliang;Zhou, Benguo;Bao, Xianxun
关键词：
-
Characterization of an RNA silencing suppressor encoded by maize yellow dwarf virus-RMV2
作者：Wang, Fang;Dong, Qing;Zhou, Benguo;Gao, Zhengliang;Zhao, Xia
关键词：RNA silencing suppressor; Polerovirus; Maize yellow dwarf virus; P0 protein; Co-infiltration assay
-
Ecogenomic survey of plant viruses infecting Tobacco by Next generation sequencing
作者：Akinyemi, Ibukun A.;Qi, Shuishui;Wu, Qingfa;Wang, Fang;Zhou, Benguo
关键词：sRNA;Bioinformatics;Assembly;Next generation sequencing;Plant virus
-
Bioinformatics analysis of amino acid sequence of acetylcholinesterase from Bemisia tabaci Genn. (Hemiptera: Aleyrodidae)
作者：Chen, Dexin;Tang, Qingfeng;Chen, Dexin;Zhou, Benguo
关键词：Acetylcholinesterase (AChE);Bemisia tabaci;sequence analysis;bioinformatics
-
Role of embryo lipoxygenase-3 under herbivore stress in stored rice
作者：Zhou, Benguo;Tang, Qingfeng
关键词：Rice;lipoxygenase-3;Rhizopertha dominica;resistance mechanisms
