文献类型： 外文期刊
作者： Yang, Yongxin 1 ; Wang, Jiaqi 1 ; Yuan, Tingjie 1 ; Bu, Dengpan 1 ; Yang, Jinhui 1 ; Sun, Peng 1 ;
作者机构： 1.Chinese Acad Agr Sci, State Key Lab Anim Nutr, Inst Anim Sci, Beijing 100193, Peoples R China
2.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Hefei 230031, Peoples R China
关键词： Animal proteomics;Cow;GeLC–MS/MS;Proteomic analysis;Ruminal epithelial tissue
期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）
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收录情况： SCI
摘要： The proteome of rumen epithelial tissue was analysed by SDS-PAGE coupled with LC-MS/MS. 813 non-redundant proteins were identified of which 7.4 % featured membrane-spanning domains and 15.4 % harboured a signal peptide. According to the gene ontology annotation, the most abundant proteins exhibited binding activities related to their molecular functions, were proteins of cellular components or belonged to various metabolic processes. A predominant group of canonical pathways in the rumen epithelial tissue was identified using the IPA software. The GeLC-MS/MS approach was used to characterise the entire protein expression repertoire in rumen tissue, providing a more detailed understanding of the important biological processes in the rumen.
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