Fusaric acid induction of programmed cell death modulated through nitric oxide signalling in tobacco suspension cells
文献类型： 外文期刊
作者： Jiao, Jiao 1 ; Zhou, Benguo 2 ; Zhu, Xiaoping 1 ; Gao, Zhengliang 2 ; Liang, Yuancun 1 ;
作者机构： 1.Shandong Agr Univ, Dept Plant Pathol, Tai An 271018, Shandong, Peoples R China
2.Anhui Acad Agr Sci, Tobacco Res Inst, Hefei 230031, Peoples R China
关键词： Caspase-3-like protease;Fusaric acid;Nitric oxide;Programmed cell death;Tobacco suspension cells
期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）
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收录情况： SCI
摘要： Fusaric acid (FA) is a nonhost-selective toxin mainly produced by Fusarium oxysporum, the causal agent of plant wilt diseases. We demonstrate that FA can induce programmed cell death (PCD) in tobacco suspension cells and the FA-induced PCD is modulated by nitric oxide (NO) signalling. Cells undergoing cell death induced by FA treatment exhibited typical characteristics of PCD including cytoplasmic shrinkage, chromatin condensation, DNA fragmentation, membrane plasmolysis, and formation of small cytoplasmic vacuoles. In addition, caspase-3-like activity was activated upon the FA treatment. The process of FA-induced PCD was accompanied by a rapid accumulation of NO in a FA dose-dependent manner. Pre-treatment of cells with NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) or NO synthase inhibitor N~G-monomethyl-arginine monoacetate (L-NMMA) significantly reduced the rate of FA-induced cell death. Furthermore, the caspase-3-like activity and the expression of PAL and Hsr203J genes were alleviated by application of cPTIO or L-NMMA to FA-treated tobacco cells. This indicates that NO is an important factor involved in the FA-induced PCD. Our results also show that pre-treatment of tobacco cells with a caspase-3-specific inhibitor, Ac-DEVD-CHO, can reduce the rate of FA-induced cell death. These results demonstrate that the FA-induced cell death is a PCD and is modulated by NO signalling through caspase-3-like activation.
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