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A molecular mechanism of resistance to streptomycin in Xanthomonas oryzae pv. oryzicola

文献类型： 外文期刊

作者： Zhang, Yong 1 ; Chen, Yu 2 ; Zhu, Xiaofeng 1 ; Xu, Ying 1 ; Hou, Yiping 1 ; Gao, Tongchun 2 ; Zhou, Mingguo 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Crop Protect, Nanjing 210095, Peoples R China

2.Anhui Acad Agr Sci, Inst Plant Protect, Hefei 230031, Peoples R China

关键词： ribosomal protein S12;streptomycin: 57-92-1;pesticide;fungicide;pUFR034 cosmid;pUFRRS: plasmid;pUFRRX: plasmid;GenBank sequence data;molecular resistance mechanism

期刊名称：PHYTOPARASITICA （ 影响因子：1.439； 五年影响因子：1.569 ）

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收录情况： SCI

摘要： Xanthomonas oryzae pv. oryzicola, the causal agent of rice leaf streak disease, was found to be sensitive to streptomycin (an aminocyclitol glycoside antibiotic), by inhibition of protein synthesis resulting from interference with translational proofreading. This study aimed to determine the molecular resistance mechanism of X. oryzae pv. oryzicola to streptomycin. Seven streptomycin-resistant mutants were obtained by UV induction or streptomycin selection. These mutants can grow at 100 mu g ml(-1) of streptomycin while the wild-type strain (RS105) cannot grow at 5 mu g ml(-1). Sequencing indicated that the rpsL gene encoding ribosomal protein S12 has 375 bp encoding 125 amino acid residues. In all resistant strains, a mutation in which AAG was substituted for AGG (Lys -> Arg) occurred either at codon 43 or 88. Two plasmids, pUFRRS and pUFRRX, were constructed by ligating the rpsL gene into the cosmid pUFR034. The plasmids pUFRRS and pUFRRX containing the Lys -> Arg mutation of the rpsL gene conferred streptomycin resistance to the sensitive wild-type strain by electroporation. Both transformants, RS1 and RS2, could grow in the medium containing 50 mu g ml(-1) of streptomycin. A mutation at codon 43 or 88 in rpsL can result in resistance of Xanthomonas oryzae pv. oryzicola to streptomycin.

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