Characteristics of the MicroRNA Expression Profile of Exosomes Released by Vero Cells Infected with Porcine Epidemic Diarrhea Virus
文献类型： 外文期刊
作者： Yin, Lei 1 ; Shen, Xuehuai 1 ; Yin, Dongdong 1 ; Wang, Jieru 1 ; Zhao, Ruihong 1 ; Dai, Yin 1 ; Pan, Xiaocheng 1 ;
作者机构： 1.Anhui Acad Agr Sci, Livestock & Poultry Epidem Dis Res Ctr Anhui Prov, Inst Anim Husb & Vet Sci, Hefei 230031, Peoples R China
2.Anhui Prov Key Lab Livestock & Poultry Prod Safet, Hefei 230031, Peoples R China
关键词： porcine epidemic diarrhea virus; exosome; microRNAs; host-pathogen interactions
期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）
ISSN：
年卷期： 2022 年 14 卷 4 期
页码：
收录情况： SCI
摘要： Exosomes are nanoscale vesicles actively secreted by a variety of cells. They contain regulated microRNA (miRNA), allowing them to function in intercellular communication. In the present study, the role of exosomal miRNAs in porcine epidemic diarrhea virus (PEDV) infection was investigated using exosomes isolated from Vero cells infected with PEDV. The results of transmission electron microscopy observation showed that the exosomes are spherical in shape, uniform in size, and negatively stained in the membrane. Nanoparticle tracking analysis showed that the average exosome particle size is 130.5 nm. The results of miRNA sequencing showed that, compared with the control group, a total of 115 miRNAs are abnormally expressed in the exosomes of infected cells. Of these, 80 miRNAs are significantly upregulated and 35 miRNAs are significantly downregulated. Functional annotation analysis showed that the differentially expressed miRNAs are associated with PEDV infection through interaction with the cAMP, Hippo, TGF-beta, HIF-1, FoxO, MAPK, and Ras signaling pathways. Thus, our findings provide important information about the effects of PEDV infection on exosomal miRNA expression and will aid the search for potential anti-PEDV drug candidates.
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