文献类型： 外文期刊
作者： Li, Juan 1 ; Chen, Like 2 ; Liang, Jing 2 ; Xu, Rongfang 1 ; Jiang, Yingli 2 ; Li, Yizhen 2 ; Ding, Jian 2 ; Li, Min 2 ; Qin, Ruiying 1 ; Wei, Pengcheng 1 ;
作者机构： 1.Anhui Acad Agr Sci, Rice Res Inst, Key Lab Rice Genet Breeding Anhui Prov, Hefei 230031, Peoples R China
2.Anhui Agr Univ, Coll Agron, Hefei 230036, Peoples R China
关键词： Prime editing; Efficiency; Engineering; Rice; Precise editing
期刊名称：GENOME BIOLOGY （ 影响因子：17.906； 五年影响因子：20.367 ）
ISSN： 1474-760X
年卷期： 2022 年 23 卷 1 期
页码：
收录情况： SCI
摘要： Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T-0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.
- 相关文献
作者其他论文 更多>>
-
Improving plant C-to-G base editors with cold-adapted glycosylase and TadA-8e variants
作者：Jiang, Yingli;Xiao, Zhi;Zhou, Suhuai;Tong, Chaoyun;Jin, Shan;Liu, Xiaoshuang;Wei, Pengcheng;Xiao, Zhi;Tong, Chaoyun;Jin, Shan;Qin, Ruiying;Xu, Rongfang;Li, Juan;Xiao, Zhi;Zhou, Suhuai;Wei, Pengcheng;Luo, Zhaopeng;Pan, Lang
关键词：
-
Identification and characterization of cold-responsive cis-element in the OsPHD13 and OsPHD52 promoter and its upstream regulatory proteins in rice
作者：Wang, Zimeng;Li, Hao;Li, Juan;Yang, Yachun;Xu, Zuntao;Yang, Jianbo;Ma, Hui;Wang, Zimeng;Wei, Pengcheng
关键词：Rice; PHD-finger transcription factor; Low temperature tolerance; Promoter region; CRT/DRE element
-
An Importin Protein SlIMPA3 Interacts with SlLCD1 and Regulates Tomato Fruit Ripening
作者：Zhang, Min;Peng, Xiang-Jun;Liu, Nan-Nan;Lu, Zi-Xu;Zhao, Yu-Qi;Yao, Gai-Fang;Hu, Kang-Di;Zhang, Hua;Li, Juan;Xu, Rong-Fang
关键词：antioxidant enzymes; fruit ripening; hydrogensulfide (H2S); importin; ROS homeostasis; tomato
-
Phosphorus Functional Genes Control Rice Yield via Microbial Biomass Phosphorus and Plant Phosphorus Uptake in a Rice-Oilseed Rape Rotation System Compared with a Rice-Wheat Rotation System
作者：Zhang, Qingyue;Yu, Weijia;Huai, Shengchang;Jin, Yuwen;Li, Guihua;Lu, Changai;Yu, Weijia;Li, Min;Cheng, Wenlong;Wu, Ji
关键词：rice-oilseed rape rotation; rice-wheat rotation; microbial biomass phosphorus; P functional genes; soil phosphorus bioavailability; rice yield
-
Pyrene-1-carboxaldehyde hydrazone modified mesoporous silica foam-based strategy for efficient and accurate electroanalysis of copper in food samples
作者：Liang, Jing;Ning, Dejiao;Yan, Feiyan;Chen, Weiwei;Xie, Liping;Wang, Yanli;Jiang, Cuiwen;Tang, Li;Li, Tao;Huang, Xiaochuan;Ya, Yu;Li, Hu;Huang, Ke-jing
关键词：Mesoporous silica foam; Organic ligand; Electrochemical sensor; Cu 2+detection; Food analysis
-
Analysis of genome-wide association studies of low-temperature germination in Xian and Geng rice
作者：Li, Kang;Hassan, Muhammad Ahmad;Gan, Quan;Lin, Cuixiang;Ten, Bin;Zhou, Kunneng;Ni, Dahu;Song, Fengshun;Li, Kang;Guo, Jinmeng;Zhao, Xueyu;Li, Min;Shi, Yingyao
关键词：rice; LTG; GWAS; QTLs; low-temperature; breeding
-
Deciphering the role of particulate organic matter in soil nitrogen transformation in rice-rapeseed and rice-wheat rotation systems
作者：Bu, Rongyan;Han, Shang;Cheng, Wenlong;Tang, Shan;Li, Min;Wang, Hui;Wu, Ji;Wu, Yue;Ge, Zibing;Wu, Ji
关键词：Labile organic matter; Organic matter bioavailability; Crop rotation; Potentially mineralizable N
