文献类型： 外文期刊
作者： Li, Juan 1 ; Chen, Like 2 ; Liang, Jing 2 ; Xu, Rongfang 1 ; Jiang, Yingli 2 ; Li, Yizhen 2 ; Ding, Jian 2 ; Li, Min 2 ; Qin, Ruiying 1 ; Wei, Pengcheng 1 ;
作者机构： 1.Anhui Acad Agr Sci, Rice Res Inst, Key Lab Rice Genet Breeding Anhui Prov, Hefei 230031, Peoples R China
2.Anhui Agr Univ, Coll Agron, Hefei 230036, Peoples R China
关键词： Prime editing; Efficiency; Engineering; Rice; Precise editing
期刊名称：GENOME BIOLOGY （ 影响因子：17.906； 五年影响因子：20.367 ）
ISSN： 1474-760X
年卷期： 2022 年 23 卷 1 期
页码：
收录情况： SCI
摘要： Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T-0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.
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