您好，欢迎访问安徽省农业科学院 机构知识库！

ArMYB89 and ArCOP1 interaction modulates anthocyanin biosynthesis in Acer rubrum leaves under low-temperature conditions

文献类型： 外文期刊

作者： Shah, Faheem Afzal 1 ; Chen, Zhu 1 ; Kamal, Khan Arif 1 ; Zhao, Yue 1 ; Zhu, Zhiyong 2 ; Chen, Jinhuan 3 ; Ren, Jie 1 ;

作者机构： 1.Anhui Acad Agr Sci, Inst Agr Engn, Hefei 2300031, Anhui, Peoples R China

2.Ningbo City Coll Vocat Technol, Ningbo 315502, Peoples R China

3.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China

关键词： Acer rubrum; anthocyanins; light; low temperature; ArMYB89, ArUGT52, ArCOP1

期刊名称：PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：7.0 ）

ISSN： 0960-7412

年卷期： 2024 年 120 卷 6 期

页码：

收录情况： SCI

摘要： Acer rubrum, a famous ornamental tree, produces bright red-coloured leaves because of the temperature decline from summer to autumn. This process's molecular mechanism is elusive, so we have investigated how anthocyanin biosynthesis is induced in A. rubrum leaves under low temperatures. The results of low-temperature treatment under light and dark conditions showed that the low-temperature promoted anthocyanin accumulation in A. rubrum is light-dependent. The transcriptome analysis showed that ArMYB89 was significantly highly expressed in leaves of A. rubrum growing under low temperatures with light conditions. The findings from the Dap-seq analysis, yeast one hybridisation, electrophoretic mobility shift assay and luciferase reporter assay indicated that the ArMYB89 transcription factor binds directly to the promoter of ArUGT52 and stimulates its transcription. The co-expression of ArUGT52 with ArMYB89 significantly induced anthocyanin levels under low temperatures with light conditions. Enzyme activity analysis showed that ArUGT52 could convert Cyanidins and Pelargonidins into Cyanidin-3-O-glucoside and Pelargonidin 3-glucoside, which are considered the main anthocyanins in red colour leaves of A. rubrum. The results of yeast two hybridisation, pulldown assay and bimolecular fluorescence complementation experiment showed an interaction between COP1 and ArMYB89, while in vivo and in vitro protein ubiquitination assay demonstrated that ArCOP1 ubiquitinates ArMYB89. Notably, co-expression of ArCOP1 with ArMYB89 significantly reduced anthocyanin levels, while the virus-induced gene silencing of ArCOP1 significantly induced anthocyanin levels under low temperatures with light conditions. In conclusion, this work revealed the molecular mechanism regulating anthocyanin accumulation in the A. rubrum leaves under low temperatures.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台