文献类型： 外文期刊
作者： Yang, Liangli 1 ; Zhao, Yue 1 ; Gan, Quan 2 ; Liang, Dan 1 ; Shu, Rui 2 ; Jiang, Song 1 ; Xie, Ruiping 1 ; Meng, Yan 1 ;
作者机构： 1.Anhui Agr Univ, Sch Life Sci, 130 West Changjiang Rd, Hefei 230036, Peoples R China
2.Anhui Acad Agr Sci, 40 Nongke South Rd, Hefei 230036, Peoples R China
3.Anhui Int Joint Res & Dev Ctr Sericulture Resourc, 130 West Changjiang Rd, Hefei 230036, Peoples R China
关键词： Bombyx mori; BmSUC1; Ser1; CRISPR; Cas9; mechanical properties
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 17 期
页码：
收录情况： SCI
摘要： BmSuc1, a novel animal-type beta-fructofuranosidase (beta-FFase, EC 3.2.1.26) encoding gene, was cloned and identified for the first time in the silkworm, Bombyx mori. BmSuc1 was specifically and highly expressed in the midgut and silk gland of Bombyx mori. Until now, the function of BmSuc1 in the silk gland was unclear. In this study, it was found that the expression changes of BmSuc1 in the fifth instar silk gland were consistent with the growth rate of the silk gland. Next, with the aid of the CRISPR/Cas9 system, the BmSuc1 locus was genetically mutated, and homozygous mutant silkworm strains with truncated beta-FFase (BmSUC1) proteins were established. BmSuc1 mutant larvae exhibited stunted growth and decreased body weight. Interestingly, the molecular weight of part of Sericin1 (Ser1) in the silk gland of the mutant silkworms was reduced. The knockout of BmSuc1 reduced the sericin content in the silkworm cocoon shell, and the mechanical properties of the mutant line silk fibers were also negatively affected. These results reveal that BmSUC1 is involved in the synthesis of Ser1 protein in silk glands and helps to maintain the homeostasis of silk protein content in silk fibers and the mechanical properties of silk fibers, laying a foundation for the study of BmSUC1 regulation of silk protein synthesis in silk glands.
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