Molecular characterization and functional analysis of AAT and SAA in Lateolabrax maculatus and Micropterus salmoides
文献类型： 外文期刊
作者： Yang, Yawen 1 ; Xie, Yushuai 1 ; Sun, Zhaosheng 1 ; Pan, Tingshuang 3 ; Qi, Zhitao 4 ; Chen, Yuxi 1 ; Gao, Qian 1 ;
作者机构： 1.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China
2.Shanghai Ocean Univ, Natl Pathogen Collect Ctr Aquat Anim, Shanghai 201306, Peoples R China
3.Anhui Acad Agr Sci, Inst Fisheries Sci, Hefei 230031, Peoples R China
4.Yancheng Inst Technol, Sch Marine & Bioengn, Yancheng 224051, Peoples R China
关键词： AAT; SAA; Acute phase protein; Antimicrobial immunity; Lateolabrax maculatus; Micropterus salmoides
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：3.9； 五年影响因子：4.2 ）
ISSN： 1050-4648
年卷期： 2025 年 166 卷
页码：
收录情况： SCI
摘要： Both alpha-1 antitrypsin (AAT) and serum amyloid A (SAA) are involved in the regulation of infection, tissue damage and inflammation. In this study, AAT and SAA genes from spotted seabass (Lateolabrax maculatus) and largemouth bass (Micropterus salmoides) were cloned and characterized, named LmAAT, MsAAT, LmSAA and MsSAA, respectively. LmAAT, MsAAT, LmSAA and MsSAA were 1664 bp, 1688 bp, 505 bp and 476 bp long, encoded 404, 409, 121 and 122 amino acid residues. These genes shared similar protein domain, high identities and closely evolutionary relationships with their teleost counterparts, separately. These four genes were expressed in all tissues tested, with the highest levels in the liver. Lipopolysaccharide (LPS) and Edwardsiella tarda significantly induced the expression of these four genes in the liver, head kidney, spleen, gills and intestine. Further analysis showed that LmAAT and MsAAT were predominantly localized to the plasma membrane of HEK-293T cells, while LmSAA and MsSAA were mainly expressed in the nucleus. Moreover, TNF alpha expression was induced by overexpression of LmAAT or MsAAT in L. maculatus brain cells (LMB). Our results lay the foundation for further investigations into the immune function of AAT and SAA in fish.
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