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Comprehensive genomic analysis and characterization of a new ST 174 type Klebsiella variicola strain isolated from chicken embryos

文献类型： 外文期刊

作者： Shen, Xuehuai 1 ; Yin, Lei 1 ; Ma, Haitian 3 ; Pan, Xiaocheng 1 ; Zhang, Danjun 1 ; Zhao, Ruihong 1 ; Dai, Yin 1 ; Hou, H 1 ;

作者机构： 1.Anhui Acad Agr Sci, Livestock & Poultry Epidem Dis Res Ctr Anhui Prov, Inst Anim Husb & Vet Sci, Hefei, Anhui, Peoples R China

2.Anhui Prov Key Lab Livestock & Poultry Prod Safet, Hefei, Anhui, Peoples R China

3.Nanjing Agr Univ, Coll Vet Med, Key Lab Anim Physiol & Biochem, Nanjing, Peoples R China

4.Anhui Acad Agr Sci, Inst Anim Husb & Vet Med, 40 Nongke South Rd, Hefei, Anhui, Peoples R China

关键词： Klebsiella variicola; Chicken embryo; Whole genome sequencing; Sequence type; Embryo lethality; Embryo hatchability

期刊名称：INFECTION GENETICS AND EVOLUTION （ 影响因子：3.342； 五年影响因子：3.188 ）

ISSN： 1567-1348

年卷期： 2021 年 90 卷

页码：

收录情况： SCI

摘要： Klebsiella variicola is a widespread opportunistic pathogen that causes infections in humans and animals. Herein a novel Klebsiella strain, AHKv-S01, was isolated and identified from dead chicken embryos in Anhui, China. Its genome contained a circular chromosome of 5,505,304 bp, with 5244 protein-coding genes, and an integrative conjugative element region containing 79 ORF sequences. AHKv-S01 was given a new sequence type number-174. Phylogenetic analyses showed that rpoB partial nucleotide sequences were highly reliable for identifying Klebsiella spp. Most of the 340 unique genes of AHKv-S01 were involved in cell envelop biogenesis, transcription, transport, and metabolic processes. Moreover, AHKv-S01 was sensitive to several antibiotics, but it showed strong resistance to penicillins, macrolides, and lincosamide. The genome contained three drug efflux pump superfamilies, beta-lactamase genes, and fosfomycin resistance-related genes. Most drug resistance genes showed amino acid mutations. Multiple virulence and pathogenic factors were also identified, and they were mainly related to adhesion, secretion, iron acquisition, and immune evasion. Chicken embryo lethality assay results revealed that the 7-day chicken embryo lethality rate was 80%, 40%, and 50% for AHKv-S01, K. pneumoniae ATCC10031, and K. pneumoniae CICC24714, respectively. The median lethal dose of AHKv-S01 was 39.9 CFU/embryo. Even low infection levels of AHKv-S01 caused a significant reduction in chicken embryo hatchability. Severe pathological changes to the liver, heart, and brain tissues of embryos infected with AHKv-S01 were observed, and these changes appeared earlier in the heart and brain than in the liver. To conclude, our results provide a foundation for further studies aiming to assess the potential risk of K. variicola to poultry populations and production yields.

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