Plasma metabolite changes in dairy cows during parturition identified using untargeted metabolomics
文献类型： 外文期刊
作者： Luo, Z. Z. 1 ; Shen, L. H. 1 ; Jiang, J. 1 ; Huang, Y. X. 1 ; Bai, L. P. 1 ; Yu, S. M. 1 ; Yao, X. P. 1 ; Ren, Z. H. 1 ; Yan 1 ;
作者机构： 1.Sichuan Agr Univ, Dept Clin Vet Med, Coll Vet Med, Chengdu 611130, Sichuan, Peoples R China
2.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Hefei 230031, Peoples R China
关键词： plasma metabolite; parturition; ultra-high-performance liquid chromatography/time-of-flight mass spectrometry; metabolic response
期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）
ISSN： 0022-0302
年卷期： 2019 年 102 卷 5 期
页码：
收录情况： SCI
摘要： The metabolic responses of cows undergo substantial changes during the transition from late pregnancy to early lactation. However, the molecular mechanisms associated with these changes in physiological metabolism have not been clearly elucidated. The objective of this study was to investigate metabolic changes in transition cows from the perspective of plasma metabolites. Plasma samples collected from 24 multiparous dairy cows on approximately d 21 prepartum and immediately postpartum were analyzed using ultra-high-performance liquid chromatography/time-of-flight mass spectrometry in positive and negative ion modes. In conjunction with multidimensional statistical methods (principal component analysis and orthogonal partial least squares discriminant analysis), differences in plasma metabolites were identified using the t-test and fold change analysis. Sixty-seven differential metabolites were identified consisting of AA, lipids, saccharides, and nucleotides. The levels of 32 plasma metabolites were significantly higher and those of 35 metabolites significantly lower after parturition than on d 21 prepartum. Pathway analysis indicated that the metabolites that increased from late pregnancy to early lactation were primarily involved in lipid metabolism and energy metabolism, whereas decreased metabolites were related to AA metabolism.
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