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Proteomic analysis of Tianzhu White Yak (Bos grunniens) testis at different sexual developmental stages

文献类型： 外文期刊

作者： Ruan, Chong-Mei 1 ; Wang, Jing 3 ; Yang, Yong-Xin 4 ; Hu, Jun-Jie 1 ; Ma, You-Ji 5 ; Zhang, Yong 1 ; Zhao, Xing-Xu 1 ;

作者机构： 1.Gansu Agr Univ, Coll Vet Med, Lanzhou, Gansu, Peoples R China

2.Xinyang Agr & Forestry Univ, Sch Anim Sci & Vet Med, Xinyang, Peoples R China

3.Hebei North Univ, Coll Anim Sci & Technol, Anim Husb Base Teaching & Res Sect, Zhangjiakou, Hebei, Peoples R China

4.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Hefei, Anhui, Peoples R China

5.Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou, Gansu, Peoples R China

关键词： proteome; sexual maturation; testis; Tianzhu White yak; two-dimensional gel electrophoresis

期刊名称：ANIMAL SCIENCE JOURNAL （ 影响因子：1.749； 五年影响因子：1.909 ）

ISSN： 1344-3941

年卷期： 2019 年 90 卷 3 期

页码：

收录情况： SCI

摘要： To explore the protein expression profiles of white yak (Bos grunniens) testis at different sexual developmental stages. The protein profiles of yak testis were determined using two-dimensional electrophoresis and the expression levels of 298 protein spots were analyzed. Mass spectrometry was performed to identify those significantly differential expressed proteins; Western blotting was used to confirm the results. During the developmental stages, 29 protein spots showed more than twofold differences (p < 0.05) at >= 1 time point and were successfully identified. Two proteins were upregulated with age (category 1), five proteins (17.2%) were downregulated with age (category 2), four proteins were upregulated before 4 years of age and downregulated thereafter (category 3), fifteen proteins were upregulated before 2 years of age and downregulated thereafter (category 4), and three proteins fluctuated with age (category 5). The expression patterns of regucalcin and heat shock 60 kDa protein in category 2 were confirmed. The 29 differentially expressed proteins from yak testes (some had more than one function) were categorized into binding (n = 15), catalytic activity (n = 13), molecular function regulator (n = 4), antioxidant (n = 4), molecular transducer (n = 2), transporter (n = 1), and structural molecule (n = 1). The identification and analysis of these testis proteins may assist in understanding the developmental biology of reproduction system in male yak.

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