文献类型： 外文期刊
作者： Zhou, Feng-Yan 1 ; Lu, Huan 1 ; Huang, Xueping 1 ; Han, Yunjing 1 ; Zhang, Yong 1 ; Han, Heping 2 ; Tsykalchuk, Liudmyla 3 ; Nyporko, Alex 3 ; Yu, Qin 2 ;
作者机构： 1.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei 230001, Peoples R China
2.Univ Western Australia, Sch Agr & Environm, Australian Herbicide Resistance Initiat AHRI, Perth, WA 6009, Australia
3.Taras Shevchenko Natl Univ Kyiv, UA-01033 Kiev, Ukraine
关键词： Raphanus raphanistrum; Mesotrione; Herbicide resistance; RrCYP72A15; RrCYP71A28
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）
ISSN： 0048-3575
年卷期： 2025 年 210 卷
页码：
收录情况： SCI
摘要： In our previous study with a wild radish (Raphanus raphanistrum) population resistant to HPPD-inhibiting herbicides we indicated that two additional candidate P450 genes, CYP71A28 and CYP72A13-like, may also contribute to the resistance. This study investigates the role of these two P450 genes in mesotrione resistance in the wild radish population. The full-length R. raphanistrum P450 genes (thereafter named as RrCYP71A28 and RrCYP72A15) were cloned from mesotrione-resistant (R) and -susceptible (S) wild radish plants. RT-qPCR results showed that basal expression levels of the two P450 genes are significantly higher (up to 3-fold) in the R than the S plants. Escherichia coli cells transformed respectively with RrCYP71A28 and RrCYP72A15 were more tolerant to mesotrione. Transgenic Arabidopsis plants expressing RrCYP72A15 showed a modest level of resistance to mesotrione. UPLC-MS/MS analysis demonstrated that tissue mesotrione levels in RrCYP72A15 transgenic Arabidopsis plants were significantly lower (up to 2-fold) than that in the wild type. Structural modelling predicts CYP72A15 can bind to RrCYP72A15 and metabolize mesotrione likely through formation of 4-OH-mesotrione. Although the RrCYP72A15 gene confers a modest level of resistance, overexpression of the multiple herbicidemetabolizing genes could contribute to the low level of mesotrione resistance observed in the R wild radish population.
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