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Genetic improvement of the shoot architecture and yield in soya bean plants via the manipulation of GmmiR156b

文献类型： 外文期刊

作者： Sun, Zhengxi 1 ; Su, Chao 2 ; Yun, Jinxia 1 ; Jiang, Qiong 2 ; Wang, Lixiang 1 ; Wang, Youning 1 ; Cao, Dong 4 ; Zhao, Fa 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China

2.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing, Peoples R China

3.Univ Chinese Acad Sci, Beijing, Peoples R China

4.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Harbin, Heilongjiang, Peoples R China

5.Hebei Acad Agr & Forestry Sci, Shijiazhuang, Hebei, Peoples R China

6.Anhui Acad Agr Sci, Hefei, Anhui, Peoples R China

7.Guangzhou Univ, Sch Life Sci, Guangzhou, Guangdong, Peoples R China

关键词： soya bean; GmmiR156b; SPL; shoot branching; plant architecture; yield

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

年卷期： 2019 年 17 卷 1 期

页码：

收录情况： SCI

摘要： The optimization of plant architecture in order to breed high-yielding soya bean cultivars is a goal of researchers. Tall plants bearing many long branches are desired, but only modest success in reaching these goals has been achieved. MicroRNA156 (miR156)-SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) gene modules play pivotal roles in controlling shoot architecture and other traits in crops like rice and wheat. However, the effects of miR156-SPL modules on soya bean architecture and yield, and the molecular mechanisms underlying these effects, remain largely unknown. In this study, we achieved substantial improvements in soya bean architecture and yield by overexpressing GmmiR156b. Transgenic plants produced significantly increased numbers of long branches, nodes and pods, and they exhibited an increased 100-seed weight, resulting in a 46%-63% increase in yield per plant. Intriguingly, GmmiR156b overexpression had no significant impact on plant height in a growth room or under field conditions; however, it increased stem thickness significantly. Our data indicate that GmmiR156b modulates these traits mainly via the direct cleavage of SPL transcripts. Moreover, we found that GmSPL9d is expressed in the shoot apical meristem and axillary meristems (AMs) of soya bean, and that GmSPL9d may regulate axillary bud formation and branching by physically interacting with the homeobox gene WUSCHEL (WUS), a central regulator of AM formation. Together, our results identify GmmiR156b as a promising target for the improvement of soya bean plant architecture and yields, and they reveal a new and conserved regulatory cascade involving miR156-SPL-WUS that will help researchers decipher the genetic basis of plant architecture.

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