文献类型： 外文期刊
作者： Gao, Qiuping 1 ; Yang, Liangli 1 ; Dai, Junjun 2 ; Yuan, Guozhen 1 ; Wang, Lei 1 ; Qian, Cen 1 ; Zhu, Baojian 1 ; Liu, Ch 1 ;
作者机构： 1.Anhui Agr Univ, Sch Life Sci, Hefei, Anhui, Peoples R China
2.Anhui Acad Agr Sci, Sericultural Res Inst, Hefei, Anhui, Peoples R China
关键词： Bombyx mori; Serpin; Immunity; Microbial infection; AMPs
期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）
ISSN： 0022-2011
年卷期： 2018 年 159 卷
页码：
收录情况： SCI
摘要： Serine protease inhibitors (Serpins) are a broadly distributed superfamily of proteins with a SERPIN domain and participate in several immune responses. In this study, a serpin-28 gene was identified in B. mori and its role in immune regulation was investigated. This gene has an open reading frame of 1065 bp that encodes a 354-amino acid residue polypeptide containing one SERPIN domain with a predicted molecular weight of 40.3 kDa. Recombinant Bmserpin-28 protein was expressed in Escherichia colt and used to raise rabbit anti-Bmserpin-28 polyclonal antibodies. Quantitative real-time PCR analysis revealed that Bmserpin-28 was expressed in all examined tissues, with maximum expression in the fat body and silk gland. Expression pattern of different developmental stages showed that the highest expression level was in the pupae, while the lowest expression level was recorded at the egg stage. After challenge with four different microorganisms (Escherichia colt, Beauveria bassiana, Micrococcus luteus and B. mori nuclear polyhedrosis virus), the expression pattern of Bmserpin-28 was investigated in fat body and haemocyte samples. A substantial upregulation of Bmserpin-28 expression level was recorded following pathogen challenge in both the tested tissues. Furthermore, RNA interference of Bmserpin-28 resulted in significant upregulation of antimicrobial peptide genes. In summary, our results indicated that Bmserpin-28 may be involved in the innate immunity of B. mori.
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