您好，欢迎访问安徽省农业科学院 机构知识库！

Molecular Cloning and Expression of Major Histocompatibility Complex Class Ia Gene of Swamp Eel (Monopterus albus)

文献类型： 外文期刊

作者： Pan, Ting-shuang 1 ; Jiang, He 1 ; Duan, Guo-qing 1 ; Hu, Yu-ting 1 ; Ling, Jun 1 ; Zhou, Hua-xing 1 ;

作者机构： 1.Anhui Acad Agr Sci, Fisheries Inst, Hefei 230031, Anhui, Peoples R China

关键词： swamp eel; MHC Ia; genomic structure; Aeromonas hydrophila; infection

期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 影响因子：0.287； 五年影响因子：0.593 ）

ISSN： 0792-156X

年卷期： 2018 年 70 卷

页码：

收录情况： SCI

摘要： Major histocompatibility complex (MHC) plays an important role in the immune response of vertebrates. In this study, we isolated MHC class Ia gene from swamp eel (Monopterus albus) by rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). In order to study the function of MHC Ia in swamp eel, tissue distribution and immune response of the MHC Ia after bacterial challenge were analyzed. All the characteristic features of MHC Ia chain structure were identified by the deduced amino acid sequence, including the leader peptide, a1, a2, and a3 domains, connecting peptide, transmembrane and cytoplasmic regions, conserved cysteines, and N-glycosylation site. The deduced amino acid sequence of the MHC Ia shares 27.3% to 69.6% similarity with those of other vertebrates. Quantitative real-time PCR (qRT-PCR) demonstrated that MHC Ia gene was ubiquitously expressed in ten tissues, comparatively higher in immune related tissues, including the liver and intestine. Challenge of M. albus with the extracellular pathogen, Aeromonas hydrophila, resulted in a significant increase in the expression of MHC Ia within 72 h after infection and these high levels were maintained for 72 h in kidneys, spleen, and skin, whereas in the intestine and liver, it was found first in the form of up-regulated and then down-regulated gene. These results demonstrated that MHC Ia gene played an important role in response to bacterial infection in M. albus.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台