Nucleotide variation in the phytoene synthase (ClPsy1) gene contributes to golden flesh in watermelon (Citrullus lanatus L.)
文献类型： 外文期刊
作者： Liu, Shi 1 ; Gao, Zhongqi 1 ; Wang, Xuezheng 1 ; Luan, Feishi 1 ; Dai, Zuyun 3 ; Yang, Zhongzhou 3 ; Zhang, Qian 4 ;
作者机构： 1.Northeast Agr Univ, Key Lab Biol & Genet Improvement Hort Crops North, Minist Agr & Rural Affairs, Harbin 150030, Peoples R China
2.Northeast Agr Univ, Hort & Landscape Architecture Coll, Harbin 150030, Peoples R China
3.Anhui Jianghuai Hort Technol Co Ltd, Hefei 230031, Peoples R China
4.Anhui Acad Agr Sci, Hort Inst, Hefei 230031, Peoples R China
期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）
ISSN： 0040-5752
年卷期：
页码：
收录情况： SCI
摘要： Key message A gene controlling golden flesh trait in watermelon was discovered and fine mapped to a 39.08 Kb region on chromosome 1 through a forward genetic strategy, and Cla97C01G008760 (annotated as phytoene synthase protein, ClPsy1 ) was recognized as the most likely candidate gene. Vitamin A deficiency is a worldwide public nutrition problem, and beta-carotene is the precursor for vitamin A synthesis. Watermelon with golden flesh (gf, which occurs due to an accumulated abundance of beta-carotene) is an important germplasm resource. In this study, a genetic analysis of segregated gf gene populations indicated that gf was controlled by a single recessive gene. BSA-seq (Bulked segregation analysis) and an initial linkage analysis placed the gf locus in a 290-Kb region on watermelon chromosome 1. Further fine mapping in a large population including over 1000 F-2 plants narrowed this region to 39.08 Kb harboring two genes, Cla97C01G008760 and Cla97C01G008770, which encode phytoene synthase (ClPsy1) and GATA zinc finger domain-containing protein, respectively. Gene sequence alignment and expression analysis between parental lines revealed Cla97C01G008760 as the best possible candidate gene for the gf trait. Nonsynonymous SNP mutations in the first exon of ClPsy1 between parental lines co-segregated with the gf trait only among individuals in the genetic population and were not related to flesh color in natural watermelon panels. Promoter sequence analysis of 26 watermelon accessions revealed two SNPs in the cis-acting element sequences corresponding to MYB and MYC2 transcription factors. RNA-seq data and qRT-PCR verification showed that two MYBs exhibited expression trends similar to that of ClPsy1 in the parental lines and may regulate the ClPsy1 expression. Further research findings indicate that the gf trait is determined not only by ClPsy1 but also by ClLCYB, ClCRTISO and ClNCED7, which play important roles in watermelon beta-carotene accumulation.
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