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Chronic heat stress inhibits glycogen synthesis through gga-miR-212-5p/GYS1 axis in the breast muscle of broilers

文献类型： 外文期刊

作者： Liu, Zhen 1 ; Liu, Yingsen 1 ; Xing, Tong 1 ; Li, Jiaolong 3 ; Zhang, Lin 1 ; Zhao, Liang 1 ; Jiang, Yun 4 ; Gao, Feng 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Anim Sci & Technol, Jiangsu Collaborat Innovat Ctr Meat Prod & Proc Qu, Key Lab Anim Origin Food Prod & Safety Guarantee J, Nanjing 210095, Peoples R China

2.Anhui Acad Agr Sci, Inst Anim Husb & Vet Med, Anhui Key Lab Livestock & Poultry Prod Safety Engn, Hefei 230031, Peoples R China

3.Inst Agroprod Proc, Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

4.Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210023, Peoples R China

关键词： chronic heat stress; broiler; gga-miR-212-5p; GYS1; glycogen

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 3 期

页码：

收录情况： SCI

摘要： Studies have demonstrated that chronic heat stress can accelerate glycolysis, decrease glycogen content in muscle, and affect muscle quality. However, the consequences of chronic heat stress on glycogen synthesis, miRNA expression in pectoralis major (PM) muscle, and its regulatory functions remain unknown. In this study, high-throughput sequencing and cell experiments were used to explore the effects of chronic heat stress on miRNA expression profiles and the regulatory mechanisms of miRNAs in glycogen synthesis under chronic heat stress. In total, 144 cocks were allocated into 3 groups: the normal control (NC) group, the heat stress (HS) group, and the pair-fed (PF) group. In total, 30 differently expressed (DE) miRNAs were screened after excluding the effect of feed intake, which were mainly related to metabolism, signal transduction, cell growth and death. Furthermore, the gga-miR-2125p/GYS1 axis was predicted to participate in glycogen synthesis through the miRNA-mRNA analysis, and a dual-luciferase reporter test assay confirmed the target relationship. Mechanistically, chronic heat stress up-regulated gga-miR-212-5p, which could inhibit the expression of GYS1 in the PM muscle. Knocking down ggamiR-212-5p alleviates the reduction of glycogen content caused by chronic heat stress; overexpression of ggamiR-212-5p can reduce glycogen content. This study provided another important mechanism for the decreased glycogen contents within the PM muscle of broilers under heat stress, which might contribute to impaired meat quality.

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