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Identification, Cloning, and Characterization of Two Acupuncture-Injury-Inducing Promoters in Rice

文献类型： 外文期刊

作者： Wang, Jianyu 1 ; Ma, Zengfeng 3 ; Fu, Dong 1 ; Wu, Yan 2 ; Zhou, Zaihui 4 ; Li, Changyan 2 ; Shen, Junhao 1 ;

作者机构： 1.Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bio, Sch Life Sci, Wuhan 430062, Peoples R China

2.Minist Agr & Rural Affairs, Hubei Acad Agr Sci, Hubei Key Lab Food Crop Germplasm & Genet Improvem, Lab Crop Mol Breeding,Food Crops Inst, Wuhan 430064, Peoples R China

3.Guangxi Acad Agr Sci, Rice Res Inst, Guangxi Key Lab Rice Genet & Breeding, State Key Lab Conservat & Utilizat Subtrop Agrobio, Nanning 530007, Peoples R China

4.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China

关键词： rice; transcriptome analysis; inducible promoters; transgenic crops; insect resistance

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 19 期

页码：

收录情况： SCI

摘要： As an important global food crop, rice is damaged by a variety of piercing-sucking pests. Identifying a broad-spectrum promoter induced by the physical signal of sucking pests and applying it to transgenic breeding to mitigate the damage caused by different sucking pests will significantly improve the efficiency of our breeding. This study compared the transcriptome changes in two rice varieties under needle-wounding stress to investigate their differential responses to mechanical damage. The results showed that the insect-susceptible variety TN1 exhibited more differentially expressed genes (DEGs) and greater changes in expression levels after needle treatment, indicating a more active internal gene regulatory network. GO and KEGG enrichment analysis further revealed that TN1 not only exhibited changes in genes related to the extracellular environment, but also mobilized more genes associated with stress response and defense. By screening the differentially expressed genes, we identified two promoters (P1 and P2) with inducible expression characteristics in both the resistant and susceptible rice varieties. These promoters were able to effectively drive the expression of the insect resistance gene OsLecRK1* and enhance the resistance of transgenic plants against the brown planthopper. This study provides promoter resources for the development of insect-resistant transgenic crops.

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