您好，欢迎访问安徽省农业科学院 机构知识库！

Identification of PbPG Involved in Pear (Pyrus bretschneideri Rehd.) Fruit Water Loss by Proteomic Analysis and Functional Exploration

文献类型： 外文期刊

作者： Ma, Na 1 ; Qi, Yongjie 1 ; Chen, Qian 1 ; Gao, Zhenghui 1 ; Xu, Yiliu 1 ;

作者机构： 1.Anhui Acad Agr Sci, Inst Hort Res, Hefei 230001, Peoples R China

2.Key Lab Hort Crop Genet Improvement & Ecophysiol, Hefei 230001, Peoples R China

关键词： Pear (Pyrus bretschneideri vs; 'Dangshansuli'); Proteomics analysis; Fruit water loss; Aquaporin; PbPG; Gene function

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.816； 五年影响因子：2.011 ）

ISSN： 0735-9640

年卷期：

页码：

收录情况： SCI

摘要： Mature pear fruit lose water rapidly, and the later the harvest, the more the water loss, severely limiting the fruit quality and economic benefits of pears. However, the molecular mechanism of pear fruit water loss is complicated and still far from being understood. In this study, the flesh of juice pear fruit (JP) and water loss pear fruit (WP) were used for proteomic analyses. Compared WP with JP, we obtained 159 differentially expressed proteins, especially cell wall metabolic and endomembrane transport proteins. Moreover, the cell membrane permeability and polygalacturonase (PG) activity were increased in WP compared with JP, illustrating that water loss of pear fruit may be related to the composition and metabolism of the cell wall. Furthermore, aquaporin coding genes AQPs and the cell wall metabolic gene PbPG were cloned, and sequence analysis indicated their important roles in fruit water loss. We then verified the function of PbPG in fruit water loss and further suggested that PbPG accelerated water loss of pear fruit was achieved by degrading cell wall polysaccharides, increasing intercellular layer and AQP-encoding gene expression. These findings will provide novel insights into the molecular mechanisms behind pear fruit water loss.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台