Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium Halomonas sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance
文献类型： 外文期刊
作者： Zhang, Jian 1 ; Wang, Pengcheng 1 ; Tian, Hongmei 1 ; Tao, Zhen 1 ; Guo, Tingting 1 ;
作者机构： 1.Anhui Acad Agr Sci, Inst Hort, Hefei 230031, Anhui, Peoples R China
2.Key Lab Genet Improvement & Ecophysiol Hort Crops, Hefei 230031, Anhui, Peoples R China
3.Anhui Agr Univ, Sch Life Sci, Hefei 230036, Anhui, Peoples R China
关键词： bacterial growth; endophytes; Halomonas sp; salt stress; transcriptome analysis
期刊名称：MICROORGANISMS （ 影响因子：4.128； ）
ISSN：
年卷期： 2020 年 8 卷 1 期
页码：
收录情况： SCI
摘要： Salt stress is an important adverse condition encountered during plant and microbe growth in terrestrial soil ecosystems. Currently, how ice plant (Mesembryanthemum crystallinum) growth-promoting endophytic bacteria (EB) cope with salt stress and regulate growth and the genes responsible for salt tolerance remain unknown. We applied RNA-Seq technology to determine the growth mechanism of the EB Halomonas sp. MC1 strain and the genes involved in salt tolerance. A total of 893 genes were significantly regulated after salt treatment. These genes included 401 upregulated and 492 downregulated genes. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes analysis revealed that the most enriched genes included those related to the outer membrane-bounded periplasmic space, ATPase activity, catabolic process, and proton transmembrane transport. The quantitative real-time polymerase chain reaction data were similar to those obtained from RNA-Seq. The MC1 strain maintained survival under salt stress by regulating cellular and metabolic processes and pyruvate metabolism pathways such as organic and carboxylic acid catabolic pathways. We highlighted the response mechanism of Halomonas sp. MC1 to fully understand the dynamics of complex salt-microbe interactions.
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