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Antioxidant and antimicrobial properties of Isodon amethystoides (Benth.) CY Wu et Hsuan leaf extracts against agriculturally important pathogenic fungi

文献类型： 外文期刊

作者： Zhao, Fenglan 1 ; Zhang, Wanjun 1 ; Meng, Xue 1 ; Yang, Xue 2 ; Yang, Lianzhi 1 ; Teng, Jingtong 1 ; Xue, Jianping 1 ; Du 1 ;

作者机构： 1.Huaibei Normal Univ, Coll Life Sci, Key Lab Resource Plant Biol Anhui Prov, Huaibei 235000, Anhui, Peoples R China

2.Anhui Acad Agr Sci, Inst Plant Protect, Lab Risk Assessment Qual & Safety Agroprod, MOA, Hefei, Anhui, Peoples R China

关键词： Antimicrobial activity; flavonoid content; Isodon amethystoides; pathogenic fungi; total phenolic content

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2019 年 33 卷 1 期

页码：

收录情况： SCI

摘要： In this study, we investigated the antioxidant and antimicrobial activities of Isodon amethystoides (Benth.) CY Wu et Hsuan leaf extracts. Leaves were subjected to extraction with acetone, chloroform, ethanol and ethyl acetate. The extraction efficiency of these four solvents ranked as follows: acetone > chloroform > ethanol > ethyl acetate; highest extraction yield of 18.27% was obtained using acetone as the solvent. Total phenolic content and flavonoid content were determined using Folin-Ciocalteau and aluminium chloride (AlCl3) methods, respectively. The acetone extract had the highest total phenolic content (146.77 mg GAE/g dry extract) and flavonoid content (81.57 mg RE/g dry extract) among the four types of extracts. Further, the antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and the antimicrobial activity was evaluated using disc-diffusion and broth microdilution assay. The strongest antioxidant activity was observed in the acetone extract with a half minimal inhibitory concentration (IC50) of 0.4024 mg/mL in the DPPH scavenging assay. The acetone and ethyl acetate extracts exhibited strong antimicrobial activity. The strongest antibacterial activity of the acetone extract was observed against Bacillus subtilis and Escherichia coli with a minimum inhibitory concentration (MIC) of 0.10 mg/mL and 0.05 mg/mL, respectively. The acetone extract had high antifungal activity against three of the six agriculturally important pathogenic fungi with MIC ranging from 0.5 mg/mL to 0.8 mg/mL. The antioxidant and antimicrobial activities may be attributed to the high total phenolic and flavonoid contents in the acetone extract. Our findings suggest I. amethystoides leaves as an important source of natural compounds with potential use in agriculture.

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