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Identification of lncRNAs by RNA Sequencing Analysis During in Vivo Pre-Implantation Developmental Transformation in the Goat

文献类型： 外文期刊

作者： Ling, Ying-hui 1 ; Zheng, Qi 1 ; Li, Yun-sheng 1 ; Sui, Meng-hua 1 ; Wu, Hao 1 ; Zhang, Yun-hai 1 ; Chu, Ming-xing 3 ; Ma 1 ;

作者机构： 1.Anhui Agr Univ, Coll Anim Sci & Technol, Hefei, Anhui, Peoples R China

2.Local Anim Genet Resources Conservat & Biobreedin, Hefei, Anhui, Peoples R China

3.Chinese Acad Agr Sci, Minist Agr, Key Lab Farm Anim Genet Resources & Germplasm Inn, Beijing, Peoples R China

4.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei, Anhui, Peoples R China

关键词： RNA-seq; Goat; long noncoding RNAs; pre-implantation development; zygotic gene activation

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN：

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： Pre-implantation development is a dynamic, complex and precisely regulated process that is critical for mammalian development. There is currently no description of the role of the long noncoding RNAs (lncRNAs) during the pre-implantation stages in the goat. The in vivo transcriptomes of oocytes (n = 3) and pre-implantation stages (n=19) at seven developmental stages in the goat were analyzed by RNA sequencing (RNA-Seq). The major zygotic gene activation (ZGA) event was found to occur between the 8- and 16-cell stages in the pre-implantation stages. We identified 5,160 differentially expressed lncRNAs (DELs) in developmental stage comparisons and functional analyses of the major and minor ZGAs. Fourteen lncRNA modules were found corresponding to specific pre-implantation developmental stages by weighted gene co-expression network analysis (WGCNA). A comprehensive analysis of the lncRNAs at each developmental transition of high correlation modules was done. We also identified lncRNA-mRNA networks and hub-lncRNAs for the high correlation modules at each stage. The extensive association of lncRNA target genes with other embryonic genes suggests an important regulatory role for lncRNAs in embryonic development. These data will facilitate further exploration of the role of lncRNAs in the developmental transformation in the pre-implantation stage.

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