文献类型： 外文期刊

作者： Liu, Shengrui ¹ ; Mi, Xiaozeng ¹ ; Zhang, Ran ² ; An, Yanlin ¹ ; Zhou, Qiying ³ ; Yang, Tianyuan ¹ ; Xia, Xiaobo ¹ ; Guo, ¹ ;

作者机构： 1.Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, 130 Changjiang West Rd, Hefei 230036, Anhui, Peoples R China

2.Anhui Acad Agr Sci, Tea Res Inst, Huangshang, Peoples R China

3.Xinyang Normal Univ, Henan Key Lab Tea Plant Biol, 237 Nanhu Rd, Xinyang 464000, Peoples R China

4.Univ Georgia, Dept Genet, Athens, GA 30602 USA

关键词： Bud dormancy; Bud burst; Caffeine biosynthesis; miRNAs; Phytohormones; Tea plant

期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）

ISSN： 0032-0935

年卷期： 2019 年 250 卷 4 期

页码：

收录情况： SCI

摘要： Main conclusion The roles of microRNA-mediated epigenetic regulation were highlighted in the bud dormancy-activity cycle, implying that certain differentially expressed miRNAs play crucial roles in apical bud burst, such as csn-miR319c/TCP2. microRNAs (miRNAs) are a class of small non-coding RNAs that regulate gene expression by targeting mRNA transcripts for cleavage or directing translational inhibition. To investigate whether miRNAs regulate bud dormancy-activation transition in tea plant, which largely affects the yield and price of tea products and adaptability of tea trees, we constructed small RNA libraries from three different periods of bud dormancy-burst transition. Through sequencing analysis, 262 conserved and 83 novel miRNAs were identified, including 118 differentially expressed miRNAs. Quantitative RT-PCR results for randomly selected miRNAs exhibited that our comprehensive analysis is highly reliable and accurate. The content of caffeine increased continuously from the endodormancy bud to flushing bud, and differentially expressed miRNAs coupling with their targets associated with bud burst were identified. Remarkably, csn-miR319c was downregulated significantly from the quiescent bud to burst bud, while its target gene CsnTCP2 (TEOSINTE BRANCHED/CYCLOIDEA/PROLIFERATING CELL FACTOR 2) displayed opposite expression patterns. Co-transformation experiment in tobacco demonstrated that csn-miR319c can significantly suppress the functions of CsnTCP2. This study on miRNAs and the recognition of target genes could provide new insights into the molecular mechanism of the bud dormancy-activation transition in tea plant.