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Proteomic Approach Analysis of Mammary Membrane Proteins Expression Profiles in Holstein Cows

文献类型： 外文期刊

作者： Yang, Yong-xin 1 ; Cao, Sui-zhong 3 ; Zhang, Yong 1 ; Zhao, Xing-xu 1 ;

作者机构： 1.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China

2.Anhui Acad Agr Sci, Inst Anim Husb & Vet Med, Hefei 230031, Peoples R China

3.Sichuan Agr Univ, Coll Anim Sci & Technol, Ya An 625014, Peoples R China

关键词： physicochernical characteristics;milk secretion;GenBank Sequence Data

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

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收录情况： SCI

摘要： To investigate host defense mechanisms for protecting the mammary gland from mastitis infection, the membrane fraction of mammary tissues from Holstein cows was purified by differential velocity centrifugation, and then the sodium dodecyl sulfate-polyacrylamid gel electrophoresis (SDS-PAGE) separated proteins were identified by ion trap mass spectrometer equipped with a Surveyor high performance liquid chromatography (HPLC) system. A total of 183 proteins were identified. Bioinformatics software was applied to analyse physicochemical characteristics of the identified proteins and to predict biochemical function. These data may provide valuable information to investigate the mechanisms of mammary gland milk secretion and infectious disease, and enable a clear identification of proteins and potential protein targets for therapies.

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